Molecular and morphological identification of larval opecoelids (Digenea: Opecoelidae) parasitising prosobranch snails in a Western Mediterranean lagoon

被引：34

作者：

Born-Torrijos, Ana ^{[1
]}

Kostadinova, Aneta ^{[2
,3
]}

Antonio Raga, Juan ^{[1
]}

Holzer, Astrid Sibylle ^{[2
]}

机构：

[1] Univ Valencia, Cavanilles Inst Biodivers & Evolutionary Biol, Sci Pk,POB 22 085, Valencia 46071, Spain

[2] Acad Sci Czech Republ, Inst Parasitol, Ctr Biol, CR-37005 Ceske Budejovice, Czech Republic

[3] Bulgarian Acad Sci, Inst Biodivers & Ecosyst Res, BU-1113 Sofia, Bulgaria

来源：

PARASITOLOGY INTERNATIONAL | 2012年 / 61卷 / 03期

关键词：

ITS rDNA; 28S rDNA; Morphology; Life-cycle; Opecoelidae; Cainocreadium labracis; Macvicaria obovata; Gibbula adansonii; Western Mediterranean; FISH ASSEMBLAGES; HELICOMETRA TREMATODA; PHYLOGENY; PLATYHELMINTHES; SPECIFICITY; COMMUNITY; HABITATS; BIOLOGY;

D O I：

10.1016/j.parint.2012.03.002

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

In a study of the digeneans parasitising molluscs in the Els Alfacs lagoon (Ebro Delta, Western Mediterranean) we found heavy infections with sporocysts emitting two types of cotylocercous cercariae in the prosobranch trochid gastropod Gibbula adansonii and with metacercariae in the prosobranch nassariid gastropod Cyclope neritea. A comparative analysis using ITS ribosomal DNA sequences from these larval stages and published sequences of 17 larval and adult opecoelid stages allowed us to elucidate the life-cycle of Macvicaria obovata and to confirm the identification of Cainocreadium labracis based on cercarial morphology. We provide molecular evidence for the identification and the first detailed morphological descriptions of the intra-molluscan larval stages of the two opecoelid species as well as partial 28S rDNA sequences to aid future studies on systematic relationships within the Opecoelidae. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：450 / 460

页数：11

共 45 条

[1] Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea [J].