Increased levels of ε and γ isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease

被引：22

作者：

Takahashi, H

Iwata, T

Kitagawa, Y

Takahashi, RH

Sato, Y

Wakabayashi, H

Takashima, M

Kido, H

Nagashima, K

Kenney, K

Gibbs, CJ

Kurata, T

机构：

[1] Natl Inst Infect Dis, Dept Pathol, Shinjuku Ku, Tokyo 1628640, Japan

[2] Univ Tokushima, Inst Enzyme Res, Div Enzyme Chem, Tokushima 770, Japan

[3] Hokkaido Univ, Sch Med, Lab Mol & Cellular Pathol, Sapporo, Hokkaido 060, Japan

[4] CREST, JST, Sapporo, Hokkaido, Japan

[5] Natl Inst Neurol Disorders & Stroke, Cent Nervous Syst Studies Lab, NIH, Bethesda, MD USA

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 1999年 / 6卷 / 06期

关键词：

D O I：

10.1128/CDLI.6.6.983-985.1999

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that epsilon and gamma isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis shelved that MAbs were more specific for native antigen than polyclonal antibodies were.

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页码：983 / 985

页数：3

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