Continuous butanol fermentation from xylose with high cell density by cell recycling system

被引：64
|
作者：
Zheng, Jin [1 ]
Tashiro, Yukihiro [2 ]
Yoshida, Tsuyoshi [1 ]
Gao, Ming [1 ]
Wang, Qunhui [3 ]
Sonomoto, Kenji [1 ,4 ]
机构：
[1] Kyushu Univ, Div Appl Mol Microbiol & Biomass Chem, Lab Microbial Technol,Dept Biosci & Biotechnol, Fac Agr,Grad Sch,Higashi Ku, Fukuoka 8128581, Japan
[2] Kyushu Univ, Div Appl Mol Microbiol & Biomass Chem, Lab Soil Microbiol,Dept Biosci & Biotechnol, Fac Agr,Grad Sch,Higashi Ku, Fukuoka 8128581, Japan
[3] Univ Sci & Technol Beijing, Dept Environm Engn, Beijing 100083, Peoples R China
[4] Kyushu Univ, Dept Funct Metab Design, Lab Funct Food Design, Bioarchitecture Ctr,Higashi Ku, Fukuoka 8128581, Japan
来源：
BIORESOURCE TECHNOLOGY | 2013年 / 129卷
关键词：
Xylose; Butanol fermentation; Continuous culture; Cell recycling; Clostridium saccharoperbutylacetonicum N1-4; CLOSTRIDIUM-BEIJERINCKII BA101; IMMOBILIZED CELLS; ETHANOL FERMENTATION; RENEWABLE BIOMASS; BUTYRIC-ACID; ACETONE; ACETOBUTYLICUM; CULTURE; SACCHAROPERBUTYLACETONICUM; ULTRAFILTRATION;
D O I：
10.1016/j.biortech.2012.11.066
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
A continuous butanol production system with high-density Clostridium saccharoperbutylacetonicum N1-4 generated by cell recycling was established to examine the characteristics of butanol fermentation from xylose. In continuous culture without cell recycling, cell washout was avoided by maintaining pH > 5.6 at a dilution rate of 0.26 h(-1), indicating pH control was critical to this experiment. Subsequently, continuous culture with cell recycling increased cell concentration to 17.4 g L-1, which increased butanol productivity to 1.20 g L-1 h(-1) at a dilution rate of 0.26 h(-1) from 0.529 g L-1 h(-1) without cell recycling. The effect of dilution rates on butanol production was also investigated in continuous culture with cell recycling. Maximum butanol productivity (3.32 g L-1 h(-1)) was observed at a dilution rate of 0.78 h(-1), approximately 6-fold higher than observed in continuous culture without cell recycling (0.529 g L-1 h(-1)). (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：360 / 365
页数：6
相关论文
共 50 条
  • [31] High-cell-density cultivation of an engineered Rhodococcus opacus strain for lipid production via co-fermentation of glucose and xylose
    Fei, Qiang
    Wewetzer, Sandra J.
    Kurosawa, Kazuhiko
    Rha, ChoKyun
    Sinskey, Anthony J.
    PROCESS BIOCHEMISTRY, 2015, 50 (04) : 500 - 506
  • [32] Synthesis gas fermentation at high cell density: How pH and hydrogen partial pressure affect productivity and product ratio in continuous fermentation
    Perret, Lukas
    Boukis, Nikolaos
    Sauer, Jorg
    BIORESOURCE TECHNOLOGY, 2024, 391
  • [33] The effect of initial cell concentration on xylose fermentation by Pichia stipitis
    Agbogbo, Frank K.
    Coward-Kelly, Guillermo
    Torry-Smith, Mads
    Wenger, Kevin
    Jeffries, Thomas W.
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2007, 137 (1-12) : 653 - 662
  • [34] Multi-stage high cell continuous fermentation for high productivity and titer
    Ho Nam Chang
    Nag-Jong Kim
    Jongwon Kang
    Chang Moon Jeong
    Jin-dal-rae Choi
    Qiang Fei
    Byoung Jin Kim
    Sunhoon Kwon
    Sang Yup Lee
    Jungbae Kim
    Bioprocess and Biosystems Engineering, 2011, 34 : 419 - 431
  • [35] Multi-stage high cell continuous fermentation for high productivity and titer
    Chang, Ho Nam
    Kim, Nag-Jong
    Kang, Jongwon
    Jeong, Chang Moon
    Choi, Jin-dal-rae
    Fei, Qiang
    Kim, Byoung Jin
    Kwon, Sunhoon
    Lee, Sang Yup
    Kim, Jungbae
    BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2011, 34 (04) : 419 - 431
  • [36] Contamination of a high-cell-density continuous bioreactor
    Domingues, L
    Lima, N
    Teixeira, JA
    BIOTECHNOLOGY AND BIOENGINEERING, 2000, 68 (05) : 584 - 587
  • [37] Continuous bacteriocin production with high cell density bioreactors
    BhugalooVial, P
    Grajek, W
    Dousset, X
    Boyaval, P
    ENZYME AND MICROBIAL TECHNOLOGY, 1997, 21 (06) : 450 - 457
  • [38] The effect of initial cell concentration on xylose fermentation by Pichia stipitis
    Frank K. Agbogbo
    Guillermo Coward-Kelly
    Mads Torry-Smith
    Kevin Wenger
    Thomas W. Jeffries
    Applied Biochemistry and Biotechnology, 2007, 137-140 : 653 - 662
  • [39] PHOTOMETRIC MEASUREMENT OF HIGH CELL-DENSITY BY CONTINUOUS DILUTION OF BROTH WITH A CIRCULATING SYSTEM
    YANO, T
    MASDUKI, A
    NISHIZAWA, Y
    JOURNAL OF FERMENTATION AND BIOENGINEERING, 1992, 74 (02): : 100 - 103
  • [40] Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose
    Sivagnanam, Kumaran
    Raghavan, Vijaya G. S.
    Shah, Manesh
    Hettich, Robert L.
    Verberkmoes, Nathan C.
    Lefsrud, Mark G.
    PROTEOME SCIENCE, 2011, 9
12345