A Kelch Domain-Containing F-Box Coding Gene Negatively Regulates Flavonoid Accumulation in Muskmelon

被引：100

作者：

Feder, Ari ^{[1
,2
]}

Burger, Joseph ^{[1
]}

Gao, Shan ^{[3
]}

Lewinsohn, Efraim ^{[1
]}

Katzir, Nurit ^{[1
]}

Schaffer, Arthur A. ^{[1
,4
]}

Meir, Ayala

Davidovich-Rikanati, Rachel ^{[1
]}

Portnoy, Vitaly ^{[1
]}

Gal-On, Amit ^{[5
]}

Fei, Zhangjun ^{[3
]}

Kashi, Yechezkel ^{[2
]}

Tadmor, Yaakov ^{[1
]}

机构：

[1] Agr Res Org, Newe Yaar Res Ctr, Cucurbit Sect, IL-3009500 Ramat Yishay, Israel

[2] Technion Israel Inst Technol, Fac Biotechnol & Food Engn, IL-32000 Haifa, Israel

[3] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA

[4] Volcani Ctr, ARO, Inst Plant Sci, IL-50250 Bet Dagan, Israel

[5] Volcani Ctr, ARO, Inst Plant Protect, IL-50250 Bet Dagan, Israel

来源：

PLANT PHYSIOLOGY | 2015年 / 169卷 / 03期

关键词：

BULKED SEGREGANT ANALYSIS; PHENYLPROPANOID BIOSYNTHESIS; AUXIN TRANSPORT; TOMATO FRUIT; EXPRESSION; PROTEINS; EFFICIENT; EVOLUTION; ANTHOCYANINS; GLYCOSIDES;

D O I：

10.1104/pp.15.01008

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The flavonoids are phenylpropanoid-derived metabolites that are ubiquitous in plants, playing many roles in growth and development. Recently, we observed that fruit rinds of yellow casaba muskmelons (Cucumis melo ` Inodorous Group') accumulate naringenin chalcone, a yellow flavonoid pigment. With RNA-sequencing analysis of bulked segregants representing the tails of a population segregating for naringenin chalcone accumulation followed by fine mapping and genetic transformation, we identified a Kelch domain-containing F-box protein coding (CmKFB) gene that, when expressed, negatively regulates naringenin chalcone accumulation. Additional metabolite analysis indicated that downstream flavonoids are accumulated together with naringenin chalcone, whereas CmKFB expression diverts the biochemical flux toward coumarins and general phenylpropanoids. These results show that CmKFB functions as a posttranscriptional regulator that diverts flavonoid metabolic flux.

引用

页码：1714 / 1726

页数：13

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