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Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex
被引:221
作者:
Ng, HH
Robert, F
Young, RA
Struhl, K
[1
]
机构:
[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
[2] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA
[3] MIT, Dept Biol, Cambridge, MA 02142 USA
关键词:
chromatin;
transcription;
RNA polymerase III;
nucleosome-remodeling;
gene expression;
microarray analysis;
D O I:
10.1101/gad.978902
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Genome-wide location analysis indicates that the yeast nucleosome-remodeling complex RSC has similar to700 physiological targets and that the Rsc1 and Rsc2 isoforms of the complex behave indistinguishably. RSC is associated with numerous tRNA promoters, suggesting that the complex is recruited by the RNA polymerase III transcription machinery. At RNA polymerase IT promoters, RSC specifically targets several gene classes, including histones, small nucleolar RNAs, the nitrogen discrimination pathway, nonfermentative carbohydrate metabolism, and mitochondrial function. At the histone HTA1/HTB1 promoter, RSC recruitment requires the Hir1 and Hir2 corepressors, and it is associated with transcriptional inactivity. in contrast, RSC binds to promoters involved in carbohydrate metabolism in response to transcriptional activation, but prior to association of the Pol IT machinery. Therefore, the RSC complex is generally recruited to Pol III promoters and it is specifically recruited to Pol 11 promoters by transcriptional activators and repressors.
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页码:806 / 819
页数:14
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