Nanoparticles (NPs) are frequently applied in biomedical applications. The use of human mesenchymal stem cells (hMSC) in biomedicine is pivotal, especially in oncology and tissue engineering. Titanium dioxide (TiO2) and zinc oxide (ZnO) NPs are interesting agents in experimental oncology and stem cells are discussed to be a potential vehicle for NPs to tumor sites. However, little is known about hazardous effects of NPs in hMSC. The aim of the present study was to analyze functional impairment of hMSC by ZnO- and TiO2-NPs. Cytotoxic effects of NPs were evaluated by the MTT-assay. Furthermore, multi-differentiation capacity, spheroid formation and migration were assessed. The immunophenotype was observed by flow cytometry. Cytotoxic effects were observed at 625 nM ZnO-NPs whereas no cytotoxicity was seen in hMSC by TiO2-NPs. The differentiation capacity of hMSC into osteogenic and adipose lineages was unchanged. A long-term period cultivation of hMSC for 3 weeks after NP exposure revealed a persistence of NPs in the cytoplasm. The migration capability was impaired whereas the ability to form spheroids was not affected. Flow cytometric analysis revealed distinct alteration of cell surface markers CD 90 and CD 73. Major functional properties of hMSC were unaffected by TiO2- and ZnO-NPs. However, restricted migration might critically influence wound healing capacity. Further information is needed to assess the clinical impact of these findings.
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King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Ahamed, Maqusood
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Akhtar, Mohd Javed
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Indian Inst Toxicol Res, Fibre Toxicol Div, Lucknow, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Akhtar, Mohd Javed
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Raja, Mohan
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Ahmad, Iqbal
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Indian Inst Toxicol Res, Fibre Toxicol Div, Lucknow, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Ahmad, Iqbal
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Siddiqui, Mohammad Kaleem Javed
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Council Sci & Technol Uttar Pradesh, Lucknow, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Siddiqui, Mohammad Kaleem Javed
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AlSalhi, Mohamad S.
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King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
AlSalhi, Mohamad S.
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Alrokayan, Salman A.
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King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
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Univ Lucknow, Dept Zool, Lucknow 226007, Uttar Pradesh, India
CSIR Indian Inst Toxicol Res, Fibre Toxicol Div, Lucknow, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Akhtar, Mohd Javed
;
Ahamed, Maqusood
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King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Ahamed, Maqusood
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Kumar, Sudhir
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Univ Lucknow, Dept Zool, Lucknow 226007, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Kumar, Sudhir
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Khan, M. A. Majeed
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King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Khan, M. A. Majeed
;
Ahmad, Javed
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King Saud Univ, Dept Zool, Coll Sci, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Ahmad, Javed
;
Alrokayan, Salman A.
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King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
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King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Ahamed, Maqusood
;
Akhtar, Mohd Javed
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h-index: 0
机构:
Indian Inst Toxicol Res, Fibre Toxicol Div, Lucknow, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Akhtar, Mohd Javed
;
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Raja, Mohan
;
Ahmad, Iqbal
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Indian Inst Toxicol Res, Fibre Toxicol Div, Lucknow, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Ahmad, Iqbal
;
Siddiqui, Mohammad Kaleem Javed
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h-index: 0
机构:
Council Sci & Technol Uttar Pradesh, Lucknow, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Siddiqui, Mohammad Kaleem Javed
;
AlSalhi, Mohamad S.
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h-index: 0
机构:
King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
AlSalhi, Mohamad S.
;
Alrokayan, Salman A.
论文数: 0引用数: 0
h-index: 0
机构:
King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
机构:
Univ Lucknow, Dept Zool, Lucknow 226007, Uttar Pradesh, India
CSIR Indian Inst Toxicol Res, Fibre Toxicol Div, Lucknow, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Akhtar, Mohd Javed
;
Ahamed, Maqusood
论文数: 0引用数: 0
h-index: 0
机构:
King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Ahamed, Maqusood
;
Kumar, Sudhir
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lucknow, Dept Zool, Lucknow 226007, Uttar Pradesh, IndiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Kumar, Sudhir
;
Khan, M. A. Majeed
论文数: 0引用数: 0
h-index: 0
机构:
King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Khan, M. A. Majeed
;
Ahmad, Javed
论文数: 0引用数: 0
h-index: 0
机构:
King Saud Univ, Dept Zool, Coll Sci, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia
Ahmad, Javed
;
Alrokayan, Salman A.
论文数: 0引用数: 0
h-index: 0
机构:
King Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi ArabiaKing Saud Univ, King Abdullah Inst Nanotechnol, Riyadh 11451, Saudi Arabia