Use of proZZ-obelin fusion protein in bioluminescent immunoassay

被引：23
|
作者：
Frank, LA
Illarionova, VA
Vysotski, ES
机构：
[1] Institute of Biophysics, Russian Academy of Sciences, Siberian Branch
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 219卷 / 02期
关键词：
D O I：
10.1006/bbrc.1996.0258
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coli by recombinant DNA techniques. The pro2Z-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 x 10(15) photons per mg of protein. (C) 1996 Academic Press, Inc.
引用
收藏
页码：475 / 479
页数：5
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