Dissecting tocopherols content in maize (Zea mays L.), using two segregating populations and high-density single nucleotide polymorphism markers

被引:37
|
作者
Xu Shutu [3 ]
Zhang Dalong [3 ]
Cai Ye [3 ]
Zhou Yi [3 ]
Shah, Trushar [4 ]
Ali, Farhan [1 ,2 ]
Li Qing [3 ]
Li Zhigang [3 ]
Wang Weidong [3 ]
Li Jiansheng [3 ]
Yang Xiaohong [3 ]
Yan Jianbing [1 ]
机构
[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China
[2] Cereal Crops Res Inst, Nowshera, Khyber Pukhtoon, Pakistan
[3] China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China
[4] Int Crops Res Inst Semi Arid Trop, Dept Bioinformat, Hyderabad, Andhra Pradesh, India
来源
BMC PLANT BIOLOGY | 2012年 / 12卷
基金
中国国家自然科学基金;
关键词
Maize; Tocopherols; QTL mapping; SYNECHOCYSTIS SP PCC-6803; QUANTITATIVE TRAIT LOCI; VITAMIN-E; HOMOGENTISATE PHYTYLTRANSFERASE; FUNCTIONAL-ANALYSIS; GENETIC DISSECTION; ASSOCIATION; METABOLISM; PLASTOQUINONE; CYANOBACTERIA;
D O I
10.1186/1471-2229-12-201
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Tocopherols, which are vitamin E compounds, play an important role in maintaining human health. Compared with other staple foods, maize grains contain high level of tocopherols. Results: Two F-2 populations (K22/CI7 and K22/Dan340, referred to as POP-1 and POP-2, respectively), which share a common parent (K22), were developed and genotyped using a GoldenGate assay containing 1,536 single nucleotide polymorphism ( SNP) markers. An integrated genetic linkage map was constructed using 619 SNP markers, spanning a total of 1649.03 cM of the maize genome with an average interval of 2.67 cM. Seventeen quantitative trait loci (QTLs) for all the traits were detected in the first map and 13 in the second. In these two maps, QTLs for different traits were localized to the same genomic regions and some were co-located with candidate genes in the tocopherol biosynthesis pathway. Single QTL was responsible for 3.03% to 52.75% of the phenotypic variation and the QTLs in sum explained23.4% to 66.52% of the total phenotypic variation. A major QTL (qc5-1/qd5-1) affecting alpha-tocopherol (alpha T) was identified on chromosome 5 between the PZA03161.1 and PZA02068.1 in the POP-2. The QTL region was narrowed down from 18.7 Mb to 5.4 Mb by estimating the recombination using high-density markers of the QTL region. This allowed the identification of the candidate gene VTE4 which encodes gamma-tocopherol methyltransferase, an enzyme that transforms gamma-tocopherol (gamma T) to alpha T. Conclusions: These results demonstrate that a few QTLs with major effects and several QTLs with medium to minor effects might contribute to the natural variation of tocopherols in maize grain. The high-density markers will help to fine map and identify the QTLs with major effects even in the preliminary segregating populations. Furthermore, this study provides a simple guide line for the breeders to improve traits that minimize the risk of malnutrition, especially in developing countries.
引用
收藏
页数:14
相关论文
共 50 条
  • [41] Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups
    Giraud, Heloise
    Bauland, Cyril
    Falque, Matthieu
    Madur, Delphine
    Combes, Valerie
    Jamin, Philippe
    Monteil, Cecile
    Laborde, Jacques
    Palaffre, Carine
    Gaillard, Antoine
    Blanchard, Philippe
    Charcosset, Alain
    Moreau, Laurence
    GENETICS, 2017, 207 (03) : 1167 - 1180
  • [42] Genome-Wide Single Nucleotide Polymorphism Discovery and the Construction of a High-Density Genetic Map for Melon (Cucumis melo L.) Using Genotyping-by-Sequencing
    Chang, Che-Wei
    Wang, Yu-Hua
    Tung, Chih-Wei
    FRONTIERS IN PLANT SCIENCE, 2017, 8
  • [43] Effects of ethylenediurea (EDU) on regulatory proteins in two maize (Zea mays L.) varieties under high tropospheric ozone phytotoxicity
    Gupta, Sunil K.
    Sharma, Marisha
    Majumder, Baisakhi
    Maurya, Vivek K.
    Deeba, Farah
    Zhang, Jiao-Lin
    Pandey, Vivek
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2020, 154 : 675 - 688
  • [44] Quantitative Trait Loci Mapping of Western Corn Rootworm (Coleoptera: Chrysomelidae) Host Plant Resistance in Two Populations of Doubled Haploid Lines in Maize (Zea mays L.)
    Bohn, Martin O.
    Marroquin, Juan J.
    Flint-Garcia, Sherry
    Dashiell, Kenton
    Willmot, David B.
    Hibbard, Bruce E.
    JOURNAL OF ECONOMIC ENTOMOLOGY, 2018, 111 (01) : 435 - 444
  • [45] Low Light Increases the Abundance of Light Reaction Proteins: Proteomics Analysis of Maize (Zea mays L.) Grown at High Planting Density
    Zheng, Bin
    Zhao, Wei
    Ren, Tinghu
    Zhang, Xinghui
    Ning, Tangyuan
    Liu, Peng
    Li, Geng
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (06)
  • [46] High-density mapping for gray leaf spot resistance using two related tropical maize recombinant inbred line populations
    Chen, Long
    Liu, Li
    Li, Ziwei
    Zhang, Yudong
    Kang, Manjit S.
    Wang, Yunyue
    Fan, Xingming
    MOLECULAR BIOLOGY REPORTS, 2021, 48 (04) : 3379 - 3392
  • [47] Optimizing Sample Size for Molecular Characterization of Open-pollinated Maize (Zea Mays L.) Varieties Using Simple Sequence Repeat Markers
    Arafayne, G.
    Menkir, A.
    Adetimirin, V. O.
    Gedil, M.
    CEREAL RESEARCH COMMUNICATIONS, 2018, 46 (04) : 569 - 579
  • [48] Construction of genetic linkage map and identification of QTLs related to agronomic traits in DH population of maize (Zea mays L.) using SSR markers
    Jae-Keun Choi
    Kyu Jin Sa
    Dae Hyun Park
    Su Eun Lim
    Si-Hwan Ryu
    Jong Yeol Park
    Ki Jin Park
    Hae-Ik Rhee
    Mijeong Lee
    Ju Kyong Lee
    Genes & Genomics, 2019, 41 : 667 - 678
  • [49] Optimizing Sample Size for Molecular Characterization of Open-pollinated Maize (Zea Mays L.) Varieties Using Simple Sequence Repeat Markers
    G. Arafayne
    A. Menkir
    V. O. Adetimirin
    M. Gedil
    Cereal Research Communications, 2018, 46 : 569 - 579
  • [50] Construction of genetic linkage map and identification of QTLs related to agronomic traits in DH population of maize (Zea mays L.) using SSR markers
    Choi, Jae-Keun
    Sa, Kyu Jin
    Park, Dae Hyun
    Lim, Su Eun
    Ryu, Si-Hwan
    Park, Jong Yeol
    Park, Ki Jin
    Rhee, Hae-Ik
    Lee, Mijeong
    Lee, Ju Kyong
    GENES & GENOMICS, 2019, 41 (06) : 667 - 678