The MAP30 protein from bitter gourd (Momordica charantia) seeds promotes apoptosis in liver cancer cells in vitro and in vivo

被引：98
作者：
Fang, Evandro Fei [1 ]
Zhang, Chris Zhi Yi [2 ,3 ]
Wong, Jack Ho [1 ]
Shen, Jia Yun [4 ,5 ]
Li, Chuan Hao [6 ]
Ng, Tzi Bun [1 ]
机构：
[1] Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
[2] Sun Yat Sen Univ, State Key Lab Oncol So China, Ctr Canc, Guangzhou 510275, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Ctr Canc, Dept Pathol, Guangzhou 510275, Guangdong, Peoples R China
[4] Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Inst Digest Dis, Hong Kong, Hong Kong, Peoples R China
[5] Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Dept Med & Therapeut, Hong Kong, Hong Kong, Peoples R China
[6] Chinese Univ Hong Kong, Fac Sci, Dept Chem, Hong Kong, Hong Kong, Peoples R China
来源：
CANCER LETTERS | 2012年 / 324卷 / 01期
关键词：
MAP30; Bitter gourd; Momordica charantia; Hepatocellular carcinoma; Apoptosis; RIBOSOME-INACTIVATING PROTEINS; ANTI-HIV; ANTITUMOR-ACTIVITY; RECOMBINANT MAP30; GAP31; PROLIFERATION; CARCINOMA; INHIBITION; EXPRESSION; SURVIVAL;
D O I：
10.1016/j.canlet.2012.05.005
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Human hepatocellular carcinoma Hop G2 cells and Hep G2-bearing mice were used as in vitro and in vivo models to assess the efficacy and safety of MAP30, a natural component from Momordica charantia, as an anticancer agent against liver cancer. Molecular studies disclosed the contribution of both caspase-8 regulated extrinsic and caspase-9 regulated intrinsic caspase cascades in MAP30-induced cell apoptosis. The antitumor potential was also effective in Hep G2-bearing nude mice. Since bitter gourd is a staple in many Asian countries, MAP30 would serve as a novel and relatively safe agent for prophylaxis and treatment of liver cancer. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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收藏
页码：66 / 74
页数：9
相关论文
共 42 条
[1]   Production of antiviral and antitumor proteins MAP30 and GAP31 in cucurbits using the plant virus vector ZYMV-AGII [J].
Arazi, T ;
Huang, PL ;
Huang, PL ;
Zhang, L ;
Shiboleth, YM ;
Gal-On, A ;
Lee-Huang, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 292 (02) :441-448
[2]   Role of poly(ADP-ribose) polymerase (PARP) cleavage in apoptosis - Caspase 3-resistant PARP mutant increases rates of apoptosis in transfected cells [J].
Boulares, AH ;
Yakovlev, AG ;
Ivanova, V ;
Stoica, BA ;
Wang, GP ;
Iyer, S ;
Smulson, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (33) :22932-22940
[3]   The activity of plant-derived antiretroviral proteins MAP30 and GAP31 against herpes simplex virus infection in vitro [J].
Bourinbaiar, AS ;
LeeHuang, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 219 (03) :923-929
[4]   Bitter Melon (Momordica charantia) Extract Suppresses Adrenocortical Cancer Cell Proliferation Through Modulation of the Apoptotic Pathway, Steroidogenesis, and Insulin-Like Growth Factor Type 1 Receptor/RAC-α Serine/Threonine-Protein Kinase Signaling [J].
Brennan, Victoria C. ;
Wang, Chiung-Min ;
Yang, Wei-Hsiung .
JOURNAL OF MEDICINAL FOOD, 2012, 15 (04) :325-334
[5]   D1 dopamine receptor mediates dopamine-induced cytotoxicity via the ERK signal cascade [J].
Chen, J ;
Rusnak, M ;
Luedtke, RR ;
Sidhu, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (38) :39317-39330
[6]   Cancer-associated PP2A Aα subunits induce functional haploinsufficiency and tumorigenicity [J].
Chen, W ;
Arroyo, JD ;
Timmons, JC ;
Possemato, R ;
Hahn, WC .
CANCER RESEARCH, 2005, 65 (18) :8183-8192
[7]   CURRENT CONCEPTS Hepatocellular Carcinoma [J].
El-Serag, Hashem B. .
NEW ENGLAND JOURNAL OF MEDICINE, 2011, 365 (12) :1118-1127
[8]   Effects of recombinant MAP30 on cell proliferation and apoptosis of human colorectal carcinoma LoVo cells [J].
Fan, Jian-Ming ;
Luo, Jun ;
Xu, Jun ;
Zhu, Sha ;
Zhang, Qiao ;
Gao, De-Fu ;
Xu, Yu-Bao ;
Zhang, Gai-Ping .
MOLECULAR BIOTECHNOLOGY, 2008, 39 (01) :79-86
[9]  
Fang EF, 2011, CURR MED CHEM, V18, P4410
[10]  
Fang EF, 2011, CURR MOL MED, V11, P417
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