Effect of warming Yang and removing blood stasis method on matrix metalloproteinases/tissue inhibitor metalloproteinases levels secreted by cultured endometrial cells from patients with endometriosis

被引：0

作者：

Huang Yanhui ^{[1
,2
,4
]}

Shen Lin ^{[2
]}

Cai Anhe ^{[3
]}

Xiao Jing ^{[4
]}

机构：

[1] Wuhan Hosp Tradit Chinese Med, Dept Gynecol, Wuhan 430010, Peoples R China

[2] Huazhong Univ Sci & Technol, Wuhan Union Hosp, Tongji Med Coll, Dept Tradit Chinese Med, Wuhan 430022, Peoples R China

[3] Guangdong Prov Hosp Chinese Med, Dept Gastroenterol, Guangzhou 510120, Guangdong, Peoples R China

[4] Guangdong Prov Hosp Chinese Med, Dept Gynecol, Guangzhou 510120, Guangdong, Peoples R China

来源：

JOURNAL OF TRADITIONAL CHINESE MEDICINE | 2015年 / 35卷 / 05期

关键词：

Endometriosis; Matrix metalloproteinases; Tissue inhibitor of metalloproteinases; Warming Yang; Removing blood stasis; TISSUE INHIBITOR; EUTOPIC ENDOMETRIUM; PROMOTER REGIONS; EXPRESSION; POLYMORPHISMS; ANGIOGENESIS; ADENOMYOSIS; SUPPRESSION; PERITONEAL; OVARIAN;

D O I：

暂无

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

OBJECTIVE: To investigate the effect of Chinese medicines using the warming Yang and removing blood stasis method on levels of matrix metalloproteinases (MMPs)/tissue inhibitor metalloproteinases (TIMPs) secreted by cultured endometrial cells from patients with endometriosis. METHODS: Ectopic and eutopic endometrial cells obtaind from 15 endometriosis patients were cultured in vitro, and divided randomly into five groups: high dose; moderate dose; low dose; nemestran; blank control. The three dose groups were treated with a decoction prepared according to the principle of warming Yang and removing blood stasis; nemestran and 0.9% NaCl were administered to the nemestran group and balnk control group, respectively. Eutopic endometrial cells obtaind from 10 hysteromyoma patients were cultured in vitro,as the normal control group,0.9% NaCI were administered to the normal control group. Cell culture supernatants were collected and levels of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), tissue inhibitor metalloproteinase-1 (TIMP-1) and tissue inhibitor metalloproteinase -2 (TIMP-2) detected by enzyme-linked immuno sorbent assay (ELISA). RESULTS: Compared with the normal control group, levels of MMP-1, MMP-2, and MMP-9 in eutopic and ectopic endometrium cell supernatants in the blank control group were increased, whereas levels of TIMP-1 and TIMP-2 were decreased (P < 0.05). Compared with the blank control group, levels of MMP-1 and MMP-2 in ectopic and eutopic endometrium cell supernatants cultured in low-dose, middle-dose, and high-dose groups were decreased, whereas levels of TIMP-1 and TIMP-2 were increased significantly (P < 0.05). CONCLUSION: The warming Yang and removing blood stasis method affects expression of MMPs and TIMPs. (C) 2015 JTCM. All rights reserved.

引用

页码：571 / 576

页数：6

共 2 条

[1] Effect of warming Yang and removing blood stasis method on matrix metalloproteinases/tissue inhibitor metalloproteinases levels secreted by cultured endometrial cells from patients with endometriosis
Huang Yanhui
Shen Lin
Cai Anhe
Xiao Jing
Journal of Traditional Chinese Medicine, 2015, 35 (05) : 571 - 576
[2] Matrix Metalloproteinase-1 and Tissue Inhibitor of Metalloproteinases-1 Levels in Severely Obese Patients: What is the Effect of Weight Loss?
Papazoglou, D.
Papatheodorou, K.
Papanas, N.
Papadopoulos, T.
Gioka, T.
Kabouromiti, G.
Kotsiou, S.
Maltezos, E.
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES, 2010, 118 (10) : 730 - 734

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