Use of NMR spectroscopy in studies of sorbitol and glucose transformation by Gluconobacter oxydans

被引:0
作者
Kitova, AE [1 ]
Reshetilov, AN
Kutyshenko, VP
Kutyshenko, AV
机构
[1] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Moscow Region, Russia
[2] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142290, Moscow Region, Russia
来源
BIOFIZIKA | 2006年 / 51卷 / 02期
关键词
NMR spectroscopy; bacterium; Gluconobacter oxydans; glucose; sorbitol; transformation;
D O I
暂无
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
NMR spectroscopy was applied for studying the products of glucose and sorbitol oxidation by cells of Gluconobacter oxydans. An analysis of H-1 NMR spectra showed that the transformation of glucose results in the formation of diketogluconic acid, and sorbitol is oxidized to sorbose. In the P-32 NMR spectra, only a signal of inorganic phosphate was detected, which accumulated in the medium as a result of cell lysis.
引用
收藏
页码:306 / 309
页数:4
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