Coenzyme Q10 protects Pc12 cells from cisplatin-induced DNA damage and neurotoxicity

被引：16

作者：

Machado, Carla da Silva ^{[1
]}

Mendonca, Leonardo Meneghin ^{[1
]}

Venancio, Vinicius de Paula ^{[1
]}

Pires Bianchi, Maria Lourdes ^{[1
]}

Greggi Antunes, Lusania Maria ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Clin Toxicol & Bromatol Anal, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, Brazil

来源：

NEUROTOXICOLOGY | 2013年 / 36卷

基金：

巴西圣保罗研究基金会;

关键词：

Ubiquinone; Neuroprotective agent; Cis-diamminedichloroplatinum II; Neuronal cell model; Genotoxicity; IN-VITRO; OXIDATIVE STRESS; VITAMIN-E; P53; Q(10); NEUROPROTECTION; GENOTOXICITY; MICRONUCLEUS; GLUTATHIONE; MECHANISMS;

D O I：

10.1016/j.neuro.2013.02.004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The purpose of this study was to investigate the neuroprotective effect of a water-soluble formulation of coenzyme Q10 (WS-CoQ10) in PC12 cells exposed to cisplatin, a chemotherapeutic agent with a dose-limiting factor due to neurotoxicity. In the cytokinesis-block micronucleus cytome assay (CBMN Cyt), WS-CoQ10 (at concentrations of 0.1, 0.5 and 1.0 mu g mL(-1)) protected PC12 cells from cisplatin-induced DNA damage (0.1 mu g mL(-1)), reducing the frequency of micronuclei (MNi) and nuclear buds (NBUDs). WS-CoQ10 did not alter the mRNA expression levels of Tp53 (at a concentration of 1.0 mu g mL(-1)) and exhibited neuroprotective activity by stimulating cisplatin-inhibited neurite outgrowth in nerve growth factor (NGF)-differentiated PC12 cells (at a concentration of 0.1 mu g mL(-1)). In conclusion, WS-CoQ10 protected the PC12 cells from cisplatin-induced DNA damage and neurotoxicity. Moreover, the neuroprotective effects of WS-CoQ10 suggest a possible application in chemotherapeutic protocols. (c) 2013 Elsevier Inc. All rights reserved.

引用

页码：10 / 16

页数：7

共 56 条

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