Preparative separation and purification of β-caryophyllene from leaf oil of Vitex negundo L. var. heterophylla (Franch.) Rehd. by high speed countercurrent chromatography

被引：11

作者：

Xie, Jianchun ^{[1
]}

Wang, Shuaibin ^{[1
]}

Sun, Baoguo ^{[1
]}

Zheng, Fuping ^{[1
]}

机构：

[1] Beijing Technol & Business Univ, Sch Chem & Environm Engn, Beijing 100037, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2008年 / 31卷 / 17期

关键词：

beta-caryophyllene; essential oil; HSCCC; Vetix negundo L. var. heterophylla (Franch.) Rehd;

D O I：

10.1080/10826070802352876

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High speed countercurrent chromatography (HSCCC) coupled with an evaporative light scatter detector (ELSD) was successfully applied to preparative isolation and purification of beta-caryophyllene from essential oil of Vetix negundo L. var. heterophylla (Franch.) Rehd. Using either n-hexane-chloroform-acetonitrile (6:2:5) or n-hexane-dichloromethane-acetonitrile (10:3:7) as the two-phase solvent system, beta-caryophyllene was separated in one run from the crude essential oil by HSCCC. However, the application of n-hexane-dichloromethane-acetonitrile (10:3:7) resulted in a higher purity and recovery of beta-caryophyllene. About 85mg of beta-caryophyllene representing 97.3% by GC, was yielded from 600 mg crude essential oil when eluted with the lower phase at a flow rate of 1.5 mL.min(-1).

引用

页码：2621 / 2631

页数：11

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