Bioengineered cardiac cell sheet grafts have intrinsic angiogenic potential

被引:152
作者
Sekiya, S [1 ]
Shimizu, T [1 ]
Yamato, M [1 ]
Kikuchi, A [1 ]
Okano, T [1 ]
机构
[1] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo 1628666, Japan
关键词
angiogenesis; vascularization; myocardial tissue graft; tissue engineering; cell sheet;
D O I
10.1016/j.bbrc.2005.12.217
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previously, we have demonstrated the long-term survival of myocardial cell sheet constructs in vivo, with microvascular network formation throughout the engineered tissues. The understanding and control of these vascularization processes are a key factor for creating thicker functional tissues. Here, we show that cardiac cell sheets express angiogenesis-related genes and form endothelial cell networks in Culture. After non-invasive harvest and stacking of cell sheets using temperature-responsive Culture dishes, these endothelial cell networks are maintained and result in neovascularization upon ill vivo transplantation. Interestingly, we also discovered that all of the graft vessels are derived from the grafts themselves and these vessels migrate to connect with the host vasculature. Finally, blood vessel formation within the grafts call be controlled by changing the ratio of endothelial cells. In conclusion, myocardial tissue grafts engineered with cell sheet technology have their own inherent potential for the in vivo neovascularization that can be regulated in vitro. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:573 / 582
页数:10
相关论文
共 37 条
[1]   TREATMENT OF DEEP CARTILAGE DEFECTS IN THE KNEE WITH AUTOLOGOUS CHONDROCYTE TRANSPLANTATION [J].
BRITTBERG, M ;
LINDAHL, A ;
NILSSON, A ;
OHLSSON, C ;
ISAKSSON, O ;
PETERSON, L .
NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (14) :889-895
[2]   Ductility enhancement of RC beams confined by steel cover plates [J].
Chen, CC ;
Tseng, CL .
JOURNAL OF THE CHINESE INSTITUTE OF ENGINEERS, 2001, 24 (01) :55-64
[3]   Pulsatile flow-induced angiogenesis -: Role of Gi Subunits [J].
Cullen, JP ;
Sayeed, S ;
Sawai, RS ;
Theodorakis, NG ;
Cahill, PA ;
Sitzmann, JV ;
Redmond, EM .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2002, 22 (10) :1610-1616
[4]   MAP kinase kinase 6-p38 MAP kinase signaling cascade regulates cyclooxygenase-2 expression in cardiac myocytes in vitro and in vivo [J].
Degousee, N ;
Martindale, J ;
Stefanski, E ;
Cieslak, M ;
Lindsay, TF ;
Fish, JE ;
Marsden, PA ;
Thuerauf, DJ ;
Glembotski, CC ;
Rubin, BB .
CIRCULATION RESEARCH, 2003, 92 (07) :757-764
[5]   AN IMMUNOHISTOLOGICAL STUDY OF THE REVASCULARIZATION PROCESS IN HUMAN-SKIN TRANSPLANTED ONTO THE NUDE-MOUSE [J].
DEMARCHEZ, M ;
HARTMANN, DJ ;
PRUNIERAS, M .
TRANSPLANTATION, 1987, 43 (06) :896-903
[6]   ANGIOGENESIS IN CANCER, VASCULAR, RHEUMATOID AND OTHER DISEASE [J].
FOLKMAN, J .
NATURE MEDICINE, 1995, 1 (01) :27-31
[7]   A cardiac myocyte vascular endothelial growth factor paracrine pathway is required to maintain cardiac function [J].
Giordano, FJ ;
Gerber, HP ;
Williams, SP ;
VanBruggen, N ;
Bunting, S ;
Ruiz-Lozano, P ;
Gu, YS ;
Nath, AK ;
Huang, Y ;
Hickey, R ;
Dalton, N ;
Peterson, KL ;
Ross, J ;
Chien, KR ;
Ferrara, N .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (10) :5780-5785
[8]   Novel approach for achieving double-layered cell sheets co-culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature-responsive culture dishes [J].
Harimoto, M ;
Yamato, M ;
Hirose, M ;
Takahashi, C ;
Isoi, Y ;
Kikuchi, A ;
Okano, T .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 2002, 62 (03) :464-470
[9]   Creation of designed shape cell sheets that are noninvasively harvested and moved onto another surface [J].
Hirose, M ;
Kwon, OH ;
Yamato, M ;
Kikuchi, A ;
Okano, T .
BIOMACROMOLECULES, 2000, 1 (03) :377-381
[10]  
Kawamoto A, 2001, CIRCULATION, V103, P634