Differential regulation of IFN-γ, TNF-α, and IL-10 production by CD4+ αβTCR+ T cells and Vδ2+ γδ T cells in response to monocytes infected with Mycobacterium tuberculosis-H37Ra

被引:47
作者
Tsukaguchi, K
de Lange, B
Boom, WH
机构
[1] Case Western Reserve Univ, Sch Med, Div Infect Dis, Dept Med, Cleveland, OH 44106 USA
[2] Univ Hosp Cleveland, Cleveland, OH 44106 USA
关键词
CD4; gamma delta T cells; Mycobacterium tuberculosis; IL-10; TNF-alpha; IFN-gamma;
D O I
10.1006/cimm.1999.1497
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Mycobacterium tuberculosis bacilli readily activate CD4(+) and gamma delta T cells. CD4(+) and gamma delta T cells were compared for their ability to regulate IFN-gamma, TNF-alpha, and IL-10 production, cytokines with significant roles in the immune response to M. tuberculosis. PBMC from healthy tuberculin positive donors were stimulated with live M. tuberculosis-H37Ra, CD4(+) and gamma delta T cells mere purified by negative selection and tested in response to autologous monocytes infected with M. tuberculosis. Both subsets produced equal amounts of secreted IFN-gamma. However, the precursor frequency of IFN-gamma secreting gamma delta T cells was half that of CD4(+) T cells, indicating that gamma delta T cells were more efficient producers of IFN-gamma than CD4(+) T cells. TNF-alpha production was markedly enhanced by addition of CD4(+) and gamma delta T cells to M. tuberculosis infected monocytes, and TNF-alpha was produced by both T cells and monocytes. No differences in TNF-alpha enhancement were noted between CD4(+) and gamma delta T cells. IL-10 production by M. tuberculosis infected monocytes was not modulated by CD4(+) or gamma delta T cells. Thus CD4(+) and gamma delta T cells had similar roles in differential regulation of IFN-gamma, TNF-alpha, and IL-10 secretion in response to M. tuberculosis infected monocytes. However, the interaction between T cells and infected monocytes differed for each cytokine. IFN-gamma production was dependent on antigen presentation and costimulators provided by monocytes. TNF-alpha levels were increased by addition of TNF-alpha produced by T cells and IL-10 production by monocytes was not modulated by CD4(+) or gamma delta T cells, (C) 1999 Academic Press.
引用
收藏
页码:12 / 20
页数:9
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