Interference and Mechanism of Dill Seed Essential Oil and Contribution of Carvone and Limonene in Preventing Sclerotinia Rot of Rapeseed

被引：49

作者：

Ma, Bingxin ^{[1
]}

Ban, Xiaoquan ^{[1
]}

Huang, Bo ^{[1
]}

He, Jingsheng ^{[1
]}

Tian, Jun ^{[1
]}

Zeng, Hong ^{[1
]}

Chen, Yuxin ^{[1
]}

Wang, Youwei ^{[1
]}

机构：

[1] Wuhan Univ, Sch Pharmaceut Sci, Inst TCM & Nat Prod, Wuhan 430072, Peoples R China

来源：

PLOS ONE | 2015年 / 10卷 / 07期

关键词：

ANETHUM-GRAVEOLENS L; ANTIFUNGAL ACTIVITY; ANTIMICROBIAL ACTIVITY; CONSTITUENTS; MONOTERPENES; PEPPERMINT; FUNGI;

D O I：

10.1371/journal.pone.0131733

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study aimed to evaluate the inhibitory effects of dill (Anethum graveolens L.) seed essential oil against Sclerotinia sclerotiorum and its mechanism of action. The antifungal activities of the two main constituents, namely carvone and limonene, were also measured. Mycelial growth and sclerotial germination were thoroughly inhibited by dill seed essential oil at the 1.00 mu L/mL under contact condition and 0.125 mu L/mL air under vapor condition. Carvone also contributed more than limonene in inhibiting the growth of S. sclerotiorum. Carvone and limonene synergistically inhibited the growth of the fungus. In vivo experiments, the essential oil remarkably suppressed S. sclerotiorum, and considerable morphological alterations were observed in the hyphae and sclerotia. Inhibition of ergosterol synthesis, malate dehydrogenase, succinate dehydrogenase activities, and external medium acidification were investigated to elucidate the antifungal mechanism of the essential oil. The seed essential oil of A. graveolens can be extensively used in agriculture for preventing the oilseed crops fungal disease.

引用

页数：15