Effect of concentrated growth factors on beagle periodontal ligament stem cells in vitro

被引：102

作者：

Yu, Bohan ^{[1
]}

Wang, Zuolin ^{[1
]}

机构：

[1] Tongji Univ, Hosp Stomatol, Ctr Implant Dent, Shanghai 200092, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2014年 / 9卷 / 01期

基金：

中国国家自然科学基金;

关键词：

concentrated growth factors; periodontal ligament stem cells; co-culture; proliferation; osteo-induction; dose-dependent effect; PLATELET-RICH PLASMA; STROMAL CELLS; BONE-FORMATION; SPINE FUSION; FIBRIN PRF; DIFFERENTIATION; TISSUE; IMPLANTS; PEPTIDE;

D O I：

10.3892/mmr.2013.1756

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Identifying a reliable and effective cytokine or growth factor group has been the focus of stem cell osteogenic induction studies. Concentrated growth factors (CGFs) as the novel generation of platelet concentrate products, appear to exhibit a superior clinical and biotechnological application potential, however, there are few studies that have demonstrated this effect. This study investigated the proliferation and differentiation of periodontal ligament stem cells (PDLSCs) co-cultured with CGFs. The rate of proliferation was analyzed by cell counting and an MTT assay. Mineralization nodule counts, alkaline phosphatase activity detection, qPCR, western blot analysis and immunohistochemistry were used to analyze mineralization effects. The results showed that CGF significantly promoted the proliferation of PDLSCs, and exhibited a dose-dependent effect on the activation and differentiation of the stem cells. The application of CGF on PDLSC proliferation and osteoinduction may offer numerous clinical and biotechnological application strategies.

引用

页码：235 / 242

页数：8

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