Production of salicylic acid precursors is a major function of phenylalanine ammonia-lyase in the resistance of arabidopsis to Peronospora parasitica

被引:6
作者
MauchMani, B [1 ]
Slusarenko, AJ [1 ]
机构
[1] UNIV ZURICH, INST PLANT BIOL, CH-8008 ZURICH, SWITZERLAND
关键词
D O I
10.2307/3870265
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arabidopsis ecotype Columbia (Col-0) seedlings, transformed with a phenylalanine ammonia-lyase 1 promoter (PALI)-beta-glucuronidase (GUS) reporter construct, were inoculated with virulent and avirulent isolates of Peronospora parasitica. The PAL1 promoter was constitutively active in the light in vascular tissue but was induced only in the vicinity of fungal structures in the incompatible interaction, A double-staining procedure was developed to distinguish between GUS activity and fungal structures, The PAL1 promoter was activated in cells undergoing lignification in the incompatible interaction in response to the pathogen. Pretreatment of the seedlings with 2-aminoindan-2-phosphonic acid (AIP), a highly specific PAL inhibitor, made the plants completely susceptible, Lignification was suppressed after AIP treatment, and surprisingly, pathogen-induced PAL1 promoter activity could not be detected. Treatment of the seedlings with 2-hydroxyphenyl-aminosulphinyl acetic acid (1,1-dimethyl ester) (OH-PAS), a cinnamyl alcohol dehydrogenase inhibitor specific for the lignification pathway, also caused a shift toward susceptibility, but the effect was not as pronounced as it was with AIP. Significantly, although OH-PAS suppressed pathogen-induced lignification, it did not suppress pathogen-induced PALI promoter activation. Salicylic acid (SA), supplied to AIP-treated plants, restored resistance and both pathogen-induced lignification and activation of the PAL1 promoter. Endogenous SA levels increased significantly in the incompatible but not in the compatible combination, and this increase was suppressed by AIP but not by OH-PAS. These results provide evidence of the central role of SA in genetically determined plant disease resistance and show that lignification per se, although providing a component of the resistance mechanism, is not the deciding factor between resistance and susceptibility.
引用
收藏
页码:203 / 212
页数:10
相关论文
共 50 条
[21]   CONVERSION OF TRANS-CINNAMIC ACID TO L-PHENYLALANINE BY PHENYLALANINE AMMONIA-LYASE [J].
CHANG, J ;
GOO, YM ;
LEE, CH ;
LEE, YY ;
KIM, KJ .
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 1994, 15 (05) :387-390
[22]   PHENYLALANINE AMMONIA-LYASE IN RELATION TO CROWN RUST RESISTANCE OF OAT LEAVES [J].
YAMAMOTO, H ;
HOKIN, H ;
TANI, T ;
KADOTA, G .
PHYTOPATHOLOGISCHE ZEITSCHRIFT-JOURNAL OF PHYTOPATHOLOGY, 1977, 90 (03) :203-211
[23]   Novel photoreactive cinnamic acid analogues to elucidate phenylalanine ammonia-lyase [J].
Hashimoto, M ;
Hatanaka, Y ;
Nabeta, K .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2000, 10 (21) :2481-2483
[24]   BACTERIAL INDUCED ACTIVATION OF AN ARABIDOPSIS PHENYLALANINE AMMONIA-LYASE PROMOTER IN TRANSGENIC TOBACCO PLANTS [J].
HUANG, Y ;
MCBEATH, JH .
PLANT SCIENCE, 1994, 98 (01) :25-35
[25]   Effects of gibberellic acid (GA3) on strawberry PAL (phenylalanine ammonia-lyase) and TAL (tyrosine ammonia-lyase) enzyme activities [J].
Montero, T ;
Mollá, E ;
Martín-Cabrejas, MA ;
López-Andréu, FJ .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1998, 77 (02) :230-234
[26]   Phytophthora canker resistance in cacao: Role of peroxidase, polyphenoloxidase and phenylalanine ammonia-lyase [J].
Okey, EN ;
Duncan, EJ ;
SirjuCharran, G ;
Sreenivasan, TN .
JOURNAL OF PHYTOPATHOLOGY, 1997, 145 (07) :295-299
[27]   RADIATION DAMAGE TO GRAPEFRUIT IN RELATION TO ETHYLENE PRODUCTION AND PHENYLALANINE AMMONIA-LYASE ACTIVITY [J].
RIOV, J ;
MONSELISE, SP ;
KAHAN, RS .
RADIATION BOTANY, 1970, 10 (03) :281-+
[28]   CINNAMIC ACID PRODUCTION AS A METHOD OF ASSAY FOR PHENYLALANINE AMMONIA-LYASE IN ACETONE POWDERS OF PHASEOLUS-VULGARIS [J].
RAHE, JE ;
KUC, J ;
CHUANG, CM .
PHYTOCHEMISTRY, 1970, 9 (05) :1009-+
[29]   Phenylalanine ammonia-lyase, a key component used for phenylpropanoids production by metabolic engineering [J].
Kong, Jian-Qiang .
RSC ADVANCES, 2015, 5 (77) :62587-62603
[30]   (E)-cinnamic acid analogues as inhibitors of phenylalanine ammonia-lyase and of anthocyanin biosynthesis [J].
Kluczyk, A ;
Szefczyk, B ;
Amrhein, N ;
Zon, J .
POLISH JOURNAL OF CHEMISTRY, 2005, 79 (03) :583-592