Quartz sand as an adsorbent for purification of extracellular glucose oxidase from Penicillium funiculosum 46.1

被引：6

作者：

Eryomin, AN

Drozhdenyuk, AP

Zhavnerko, GK

Semashko, TV

Mikhailova, RV

机构：

[1] Natl Acad Sci Belarus, Inst Bioorgan Chem, Minsk 220141, BELARUS

[2] Natl Acad Sci Belarus, Inst Chem New Mat, Minsk 220141, BELARUS

[3] Natl Acad Sci Belarus, Inst Microbiol, Minsk 220141, BELARUS

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2004年 / 40卷 / 02期

关键词：

D O I：

10.1023/B:ABIM.0000018918.34739.ae

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A procedure for purification of extracellular glucose oxidase (GO, EC 1.1.3.4) from a filtrate of culture liquid (CLF) of the fungus Penicillium funiculosum 46.1 has been developed using alluvial quartz sand as an adsorbent. Modifying the sand by changing the charge and polarity did not lead to a significant increase in its adsorption capacity towards GO. The effectiveness of sand and aluminum oxide used as adsorbents for GO isolation from CLF has been compared. Glucose oxidase isolated from CLF by adsorption on sand exhibited a greater catalytic activity than enzyme preparations obtained by column chromatography on CLE Glucose oxidase from P. funiculosum 46.1 was adsorbed on sand more effectively than on aluminum oxide. It is concluded that sand may be used for fractionation of partially purified GO.

引用

页码：151 / 157

页数：7

共 18 条

[1] CALCIUM TARTRATE GEL [J].