Combined optical coherence and two-photon excitation microscopy

被引：0

作者：

Beaurepaire, E ^{[1
]}

Moreaux, L ^{[1
]}

Mertz, J ^{[1
]}

机构：

[1] Ecole Super Phys & Chim Ind Ville Paris, Lab Neurophysiol & Nouvelles Microscopies, INSERM EPI 00 02, F-75005 Paris, France

来源：

PROCEEDINGS OF INTER-INSTITUTE WORKSHOP ON IN VIVO OPTICAL IMAGING AT THE NIH | 2000年

关键词：

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-excited fluorescence (TPEF) microscopy. Independent contrast mechanisms derived from fluorescence and backscattered light provide complementary information on biological tissues. The coupling of OCM and TPEF microscopy is natural in that the two imaging modes may be run off the same laser source. The confocal arrangement for OCM provides a sub-micrometer resolution compatible with TPEF microscopy.

引用

收藏

页码：43 / 48

页数：6

相关论文

共 10 条

[1] Combined scanning optical coherence and two-photon-excited fluorescence microscopy [J].

Beaurepaire, E ;

Moreaux, L ;

Amblard, F ;

Mertz, J .

OPTICS LETTERS, 1999, 24 (14) :969-971

[2] A NUCLEAR GFP THAT MARKS NUCLEI IN LIVING DROSOPHILA EMBRYOS - MATERNAL SUPPLY OVERCOMES A DELAY IN THE APPEARANCE OF ZYGOTIC FLUORESCENCE [J].

DAVIS, I ;

GIRDHAM, CH ;

OFARRELL, PH .

DEVELOPMENTAL BIOLOGY, 1995, 170 (02) :726-729

[3] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

[4] OPTICAL COHERENCE TOMOGRAPHY [J].

HUANG, D ;

SWANSON, EA ;

LIN, CP ;

SCHUMAN, JS ;

STINSON, WG ;

CHANG, W ;

HEE, MR ;

FLOTTE, T ;

GREGORY, K ;

PULIAFITO, CA ;

FUJIMOTO, JG .

SCIENCE, 1991, 254 (5035) :1178-1181

[5] OPTICAL COHERENCE MICROSCOPY IN SCATTERING MEDIA [J].

IZATT, JA ;

HEE, MR ;

OWEN, GM ;

SWANSON, EA ;

FUJIMOTO, JG .

OPTICS LETTERS, 1994, 19 (08) :590-592

[6] Optical coherence tomography and microscopy in gastrointestinal tissues [J].

Izatt, JA ;

Kulkarni, MD ;

Wang, HW ;

Kobayashi, K ;

Sivak, MV .

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 1996, 2 (04) :1017-1028

[7] Comparative study of confocal and heterodyne microscopy for imaging through scattering media [J].

Kempe, M ;

Rudolph, W ;

Welsch, E .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1996, 13 (01) :46-52

[8] Molecular photodynamics involved in multi-photon excitation fluorescence microscopy [J].

Mertz, J .

EUROPEAN PHYSICAL JOURNAL D, 1998, 3 (01) :53-66

[9]

SAINTJALMES H, IN PRESS HDB OPTICAL

[10]

SCHMITT JM, 1993, P SOC PHOTO-OPT INS, V1889, P197, DOI 10.1117/12.155715