Effect of methyl jasmonate on the ginsenoside content of Panax ginseng adventitious root cultures and on the genes involved in triterpene biosynthesis

被引：38

作者：

Wang, Juan ^{[1
,2
]}

Gao, Wenyuan ^{[1
,2
]}

Zuo, Beimei ^{[2
]}

Zhang, Liming ^{[2
]}

Huang, Luqi ^{[3
]}

机构：

[1] Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin Key Lab Modern Drug Delivery & High Effic, Tianjin 300072, Peoples R China

[2] Tianjin Univ Sci & Technol, Coll Biotechnol, Minist Educ, Key Lab Ind Microbiol, Tianjin 300457, Peoples R China

[3] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

来源：

RESEARCH ON CHEMICAL INTERMEDIATES | 2013年 / 39卷 / 05期

关键词：

Adventitious root; Ginsenoside; Methyl jasmonate; Panax ginseng; Triterpene biosynthesis; SUSPENSION-CULTURES; QUINQUEFOLIUM; SAPONIN; GROWTH;

D O I：

10.1007/s11164-012-0730-7

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Methyl jasmonate (MJ)-induced changes of triterpene saponins in Panax ginseng C.A. Meyer adventitious roots, and expression of the genes involved in triterpene biosynthesis, were analyzed. Compared with controls, expression of the squalene epoxidase and dammarenediol synthase genes was clearly upregulated and total saponin content increased in response to MJ. The highest total saponin content was obtained by use of 10 mg L-1 MJ for 24 h, and was 4.76-fold greater than in the control group. Expression of the two genes associated with the cytochrome P450 family was no different from that in controls. The level of ginsenoside in the Rg group thus increased much less than that in the Rb group. This investigation showed that the total saponin content of ginseng adventitious root is related to gene expression in ginsenoside biosynthesis.

引用

页码：1973 / 1980

页数：8

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