Molecular study of thiol-mediated DNA radioprotection

被引：0

作者：

Savoye, C

Swenberg, C

Sabattier, R

Charlier, M

SpotheimMaurizot, M

机构：

[1] ARMED FORCES RADIOBIOL RES INST,APPL CELLULAR RADIOBIOL DEPT,BETHESDA,MD 20889

[2] CTR HOSP REG ORLEANS,SERV ONCOL & RADIOTHERAPIE,F-45067 ORLEANS,FRANCE

来源：

JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE | 1997年 / 94卷 / 02期

关键词：

radioprotection; WR agents; DNA radiosensitivity; sequence specificity;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We compare the effect of four ligands on the radiolysis of a plasmid DNA with fast neutrons. Three aminothiols, WR-1065, WR-151326 and cysteamine are studied and compared to the putrescine. We show the influence of the charge and the chemical structure of the ligand on their radioprotective ability. The putrescine, which acts only as a OH. scavenger, is less protective than the three thiols, since their SH function is involved in the ''chemical repair'' of the DNA damages. Among these thiols, the cysteamine is less protective than WR-1065, which is less efficient than WR-151326. The protection of a 80 base pairs DNA fragment by the molecules with Z=+2 is sequence-modulated and varies with the ligand and its concentration.

引用

页码：337 / 341

页数：5