Identification of a 3-gene model as a powerful diagnostic tool for the recognition of ALK-negative anaplastic large-cell lymphoma

被引：92

作者：

Agnelli, Luca ^{[2
,3
]}

Mereu, Elisabetta ^{[1
]}

Pellegrino, Elisa ^{[1
]}

Limongi, Tania ^{[1
]}

Kwee, Ivo ^{[4
,5
]}

Bergaggio, Elisa ^{[1
]}

Ponzoni, Maurilio ^{[6
,7
]}

Zamo, Alberto ^{[8
]}

Iqbal, Javeed ^{[9
]}

Piccaluga, Pier Paolo ^{[10
]}

Neri, Antonino ^{[2
,3
]}

Chan, Wing C. ^{[9
]}

Pileri, Stefano ^{[10
]}

Bertoni, Francesco ^{[4
,11
]}

Inghirami, Giorgio ^{[1
,12
,13
]}

Piva, Roberto ^{[1
,12
,13
]}

机构：

[1] Univ Turin, Dept Mol Biotechnol & Hlth Sci, Ctr Expt Res & Med Studies, I-10126 Turin, Italy

[2] Osped Maggiore Policlin, Fdn Ist Ricovero & Cura Carattere Sci Ca Granda, Milan, Italy

[3] Univ Milan, Dept Clin Sci & Community Hlth, Milan, Italy

[4] Oncol Res Inst, Lymphoma & Genom Res Program, Bellinzona, Switzerland

[5] Dalle Molle Inst Artificial Intelligence, Manno, Switzerland

[6] Ist Sci San Raffaele, Pathol Unit, I-20132 Milan, Italy

[7] Ist Sci San Raffaele, Lymphoid Malignancies Unit, I-20132 Milan, Italy

[8] Univ Verona, Dept Pathol, I-37100 Verona, Italy

[9] Univ Nebraska Med Ctr, Dept Pathol & Microbiol, Omaha, NE USA

[10] Univ Bologna, Inst Hematol & Med Oncol, S Orsola Malpighi Hosp, Bologna, Italy

[11] Oncol Inst So Switzerland, Lymphoma Unit, Bellinzona, Switzerland

[12] NYU, Sch Med, Ctr Canc, New York, NY USA

[13] NYU, Sch Med, Dept Pathol, New York, NY USA

来源：

BLOOD | 2012年 / 120卷 / 06期

关键词：

PERIPHERAL T-CELL; GENE-EXPRESSION ANALYSIS; KINASE; CLASSIFICATION; ABERRATIONS; PROFILE;

D O I：

10.1182/blood-2012-01-405555

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Anaplastic large-cell lymphomas (ALCLs) are a group of clinically and biologically heterogeneous diseases including the ALK(+) and ALK(-) systemic forms. Whereas ALK(+) ALCLs are molecularly characterized and can be readily diagnosed, specific immunophenotypic or genetic features to define ALK(-) ALCL are missing, and their distinction from other T-cell non-Hodgkin lymphomas (T-NHLs) remains controversial. In the present study, we undertook a transcriptional profiling meta-analysis of 309 cases, including ALCL and other primary T-NHL samples. Pathway discovery and prediction analyses defined a minimum set of genes capable of recognizing ALK(-) ALCL. Application of quantitative RT-PCR in independent datasets from cryopreserved and formalin-fixed paraffin-embedded samples validated a 3-gene model (TNFRSF8, BATF3, and TMOD1) able to successfully separate ALK(-) ALCL from peripheral T-cell lymphoma not otherwise specified, with overall accuracy near 97%. In conclusion, our data justify the possibility of translating quantitative RT-PCR protocols to routine clinical settings as a new approach to objectively dissect T-NHL and to select more appropriate therapeutic protocols. (Blood. 2012;120(6):1274-1281)

引用

页码：1274 / 1281

页数：8

共 33 条

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