Highly sensitive and selective miRNA detection based on a closed ring probe and multiple signal amplification

被引：25
作者：
Tang, Yaqin
He, Xiao
Zhou, Zhenxia
Tang, Jiakun
Guo, Rong
Feng, Xuli [1 ]
机构：
[1] Chongqing Univ, Sch Pharmaceut Sci, Chongqing 401331, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 96期
基金：
中国国家自然科学基金;
关键词：
CATIONIC CONJUGATED POLYMER; FLUORESCENCE DETECTION; AMPLIFIED DETECTION; LIVING CELLS; MICRORNA; DNA; MOLECULES; BIOSENSOR; STEP;
D O I：
10.1039/c6cc07719f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A highly sensitive and selective miRNA detection approach based on a small circular probe and dual signal amplification has been reported. We successfully applied the assay for quantitative let-7a detection in cell lysates and the results agreed well with those of qRT-PCR.
引用
收藏
页码：13905 / 13908
页数：4
相关论文
共 40 条
[1]   MicroRNA functions [J].
Bushati, Natascha ;
Cohen, Stephen M. .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2007, 23 :175-205
[2]   MicroRNA silencing for cancer therapy targeted to the tumour microenvironment [J].
Cheng, Christopher J. ;
Bahal, Raman ;
Babar, Imran A. ;
Pincus, Zachary ;
Barrera, Francisco ;
Liu, Connie ;
Svoronos, Alexander ;
Braddock, Demetrios T. ;
Glazer, Peter M. ;
Engelman, Donald M. ;
Saltzman, W. Mark ;
Slack, Frank J. .
NATURE, 2015, 518 (7537) :107-+
[3]   Highly Sensitive Determination of microRNA Using Target-Primed and Branched Rolling-Circle Amplification [J].
Cheng, Yongqiang ;
Zhang, Xian ;
Li, Zhengping ;
Jiao, Xiaoxia ;
Wang, Yucong ;
Zhang, Yali .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (18) :3268-3272
[4]   Potential pitfalls in microRNA profiling [J].
Chugh, Pauline ;
Dittmer, Dirk P. .
WILEY INTERDISCIPLINARY REVIEWS-RNA, 2012, 3 (05) :601-616
[5]   Analytical aspects of microRNA in diagnostics: A review [J].
de Planell-Saguer, Mariangels ;
Celina Rodicio, Maria .
ANALYTICA CHIMICA ACTA, 2011, 699 (02) :134-152
[6]   A Cross-Platform Comparison of Affymetrix and Agilent Microarrays Reveals Discordant miRNA Expression in Lung Tumors of c-Raf Transgenic Mice [J].
Del Vescovo, Valerio ;
Meier, Tatiana ;
Inga, Alberto ;
Denti, Michela Alessandra ;
Borlak, Juergen .
PLOS ONE, 2013, 8 (11)
[7]   Toehold-initiated Rolling Circle Amplification for Visualizing Individual MicroRNAs In Situ in Single Cells [J].
Deng, Ruijie ;
Tang, Longhua ;
Tian, Qianqian ;
Wang, Ying ;
Lin, Lei ;
Li, Jinghong .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 53 (09) :2389-2393
[8]   Label-free high-throughput microRNA expression profiling from total RNA [J].
Duan, Demin ;
Zheng, Ke-xiao ;
Shen, Ye ;
Cao, Rong ;
Jiang, Li ;
Lu, Zhuoxuan ;
Yan, Xiyun ;
Li, Jiong .
NUCLEIC ACIDS RESEARCH, 2011, 39 (22) :e154
[9]   Cationic Conjugated Polymers for Optical Detection of DNA Methylation, Lesions, and Single Nucleotide Polymorphisms [J].
Duan, Xinrui ;
Liu, Libing ;
Feng, Fude ;
Wang, Shu .
ACCOUNTS OF CHEMICAL RESEARCH, 2010, 43 (02) :260-270
[10]   Dual-amplified sensitive DNA detection based on conjugated polymers and recyclable autocatalytic hybridization of DNA [J].
Feng, Xuli ;
Liu, Libing ;
Yang, Qiong ;
Wang, Shu .
CHEMICAL COMMUNICATIONS, 2011, 47 (20) :5783-5785
1234