Quantitative proteomic analysis to discover potential diagnostic markers and therapeutic targets in human renal cell carcinoma

被引：37

作者：

Okamura, Noboru ^{[1
]}

Masuda, Taro ^{[2
]}

Gotoh, Akinobu ^{[3
]}

Shirakawa, Toshiro ^{[4
]}

Terao, Shuji ^{[3
]}

Kaneko, Naoki ^{[2
,5
]}

Suganuma, Kazuki ^{[2
]}

Watanabe, Makoto ^{[2
,5
]}

Matsubara, Toshiya ^{[2
,5
]}

Seto, Ryota ^{[6
]}

Matsumoto, Jun ^{[6
]}

Kawakami, Megumi ^{[6
]}

Yamamori, Motohiro ^{[6
]}

Nakamura, Tsutomu ^{[1
,6
]}

Yagami, Tatsurou ^{[7
]}

Sakaeda, Toshiyuki ^{[6
,8
]}

Fujisawa, Masato ^{[4
]}

Nishimura, Osamu ^{[2
,5
]}

Okumura, Katsuhiko ^{[1
,6
,7
]}

机构：

[1] Kobe Univ, Dept Clin Evaluat Pharmacotherapy, Grad Sch Med, Kobe, Hyogo 6500047, Japan

[2] Shimadzu Co Ltd, Life Sci Lab, Kyoto, Japan

[3] Hyogo Coll Med, Inst Adv Med Sci, Nishinomiya, Hyogo, Japan

[4] Kobe Univ, Dept Organ Therapeut, Grad Sch Med, Div Urol, Kobe, Hyogo 6500047, Japan

[5] Osaka Univ, Div Dis Prote, Inst Prot Res, Osaka, Japan

[6] Kobe Univ, Dept Hosp Pharm, Sch Med, Kobe, Hyogo 6500047, Japan

[7] Himeji Dokkyo Univ, Fac Pharmaceut Sci, Himeji, Hyogo, Japan

[8] Kyoto Univ, Frontier Educ Ctr, Grad Sch Pharmaceut Sci, Kyoto, Japan

来源：

PROTEOMICS | 2008年 / 8卷 / 15期

关键词：

biomarker; NBS method; renal cell carcinoma;

D O I：

10.1002/pmic.200700619

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Renal cell carcinoma (RCC) is relatively resistant to chemotherapy and radiotherapy. Recent advances in drug development are providing novel agents for the treatment of RCC, but the effects are still minimal. In addition, there is an urgent need to identify diagnostic markers for RCC. In this report, to discover potential diagnostic markers and therapeutic targets, we subjected RCC samples to a quantitative proteomic analysis utilizing 2-nitrobenzenesulfenyl (NBS) reagent. Proteins were extracted from RCC and adjacent normal tissue, obtained surgically from patients, and labeled with NBS reagent containing six C-12 or C-13. This was followed by trypsin digestion and the enrichment of labeled peptides. Samples were then subjected to analysis by MALDI-TOF MS. NBS-labeled peptides with a 6 Da difference were identified by MS/MS. Thirty-four proteins were upregulated in more than 60% of the patients of which some were previously known, and some were novel. The identity of a few proteins was confirmed by Western blotting and quantitative real time RT-PCR. The results suggest that NBS-based quantitative proteomic analysis is useful for discovering diagnostic markers and therapeutic targets for RCC.

引用

页码：3194 / 3203

页数：10

共 34 条

[1] Renal cell carcinoma: review of novel single-agent therapeutics and combination regimens [J].

Amato, RJ .

ANNALS OF ONCOLOGY, 2005, 16 (01) :7-15

[2] Determination of structural and functional overlap/divergence of five proto-type galectins by analysis of the growth-regulatory interaction with ganglioside GM1 in silico and in vitro on human neuroblastoma cells [J].

André, S ;

Kaltner, H ;

Lensch, M ;

Russwurm, R ;

Siebert, HC ;

Fallsehr, C ;

Tajkhorshid, E ;

Heck, AJR ;

Doeberitz, MV ;

Gabius, HJ ;

Kopitz, J .

INTERNATIONAL JOURNAL OF CANCER, 2005, 114 (01) :46-57

[3]

BART J, 2005, DIABETES, V54, P2320

[4]

CAGNEY G, 2003, PROTEOME SCI, V13, P5

[5] Galectin-1: a small protein with major functions [J].

Camby, Isabelle ;

Le Mercier, Marie ;

Lefranc, Florence ;

Kiss, Robert .

GLYCOBIOLOGY, 2006, 16 (11) :137R-157R

[6] Galectin-1 augments Ras activation and diverts Ras signals to Raf-1 at the expense of phosphoinositide 3-kinase [J].

Elad-Sfadia, G ;

Haklai, R ;

Ballan, E ;

Gabius, HJ ;

Kloog, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (40) :37169-37175

[7] Housekeeping proteins: A preliminary study illustrating some limitations as useful references in protein expression studies [J].

Ferguson, RE ;

Carroll, HP ;

Harris, A ;

Maher, ER ;

Selby, PJ ;

Banks, RE .

PROTEOMICS, 2005, 5 (02) :566-571

[8] Galectin-1 interacts with the α5β1 fibronectin receptor to restrict carcinoma cell growth via induction of p21 and p27 [J].

Fischer, C ;

Sanchez-Ruderisch, H ;

Welzel, M ;

Wiedenmann, B ;

Sakai, T ;

André, S ;

Gabius, HJ ;

Khachigian, L ;

Detjen, KM ;

Rosewicz, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (44) :37266-37277

[9]

Gabius HJ, 2002, ANTICANCER RES, V22, P405

[10] Recent progress in the management of advanced renal cell carcinoma [J].

Garcia, Jorge A. ;

Rini, Brian I. .

CA-A CANCER JOURNAL FOR CLINICIANS, 2007, 57 (02) :112-125

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