Triptolide inhibits colon-rectal cancer cells proliferation by induction of G1 phase arrest through upregulation of p21

被引：53

作者：

Liu, Juanjuan ^{[1
]}

Shen, Min ^{[2
]}

Yue, Zhenggang ^{[1
]}

Yang, Zhifu ^{[3
]}

Wang, Meng ^{[1
]}

Li, Chen ^{[1
]}

Xin, Chunyan ^{[1
]}

Wang, Yukun ^{[1
]}

Mei, Qibing ^{[1
]}

Wang, Zhipeng ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Key Lab Gastrointestinal Pharmacol Chinese Mat Me, State Adm Tradit Chinese Med, Dept Pharmacol,Sch Pharm, Xian 710032, Peoples R China

[2] Fourth Mil Med Univ, Dept Cardiovasc Dis, Xijing Hosp, Xian 710032, Peoples R China

[3] Fourth Mil Med Univ, Dept Pharmaceut, Tangdu Hosp, Xian 710032, Peoples R China

来源：

PHYTOMEDICINE | 2012年 / 19卷 / 8-9期

基金：

中国国家自然科学基金;

关键词：

Cell cycle; Colon-rectal cancer; Triptolide; Tripterygium wilfordii; p21; PROTEIN-KINASE; CYCLIN-A; ACTIVATION; EXPRESSION; APOPTOSIS; PATHWAY; LINES; GENE; AKT; MEK;

D O I：

10.1016/j.phymed.2012.02.014

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Triptolide, a diterpene triepoxide compound extracted from the traditional Chinese medicine herb Tripterygium wilfordii Hook F., is a potential cancer chemotherapeutic for tumors. However, the mechanism of anti-proliferative mechanism of triptolide in colon cancer cells is not entirely clear. Triptolide markedly inhibited HT29 and SW480 cells proliferation in a dose- and time-dependent manner. Triptolide decreased ERR and ART phosphorylation, and GABP alpha expression in colon cancer cells. Beta-catenin expression and phosphorylation were not altered by incubation of triptolide. However, we found that triptolide repressed expression of LEF/TCF. Although it did not significantly affect cells apoptosis, triptolide induced G1 phase arrest dose-dependently. Further detection for the expression of cell cycle-related proteins suggesting that triptolide stimulate expression of p21 and repress cyclin Al. Increased p21 binded to CDK4/CDK6, therefore blocked function of CDK4/CDK6, and subsequently contribute to the G1 arrest. These data suggested that triptolide is a potential agent for treatment of colon cancer, and its anti-proliferation effect mainly occur through G1 phase arrest. (C) 2012 Elsevier GmbH. All rights reserved.

引用

页码：756 / 762

页数：7

共 24 条

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