Engineered riboswitches: Expanding researchers' toolbox with synthetic RNA regulators

被引：110

作者：

Wittmann, Alexander ^{[1
]}

Suess, Beatrix ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Inst Mol Biosci, D-60438 Frankfurt, Germany

来源：

FEBS LETTERS | 2012年 / 586卷 / 15期

关键词：

Synthetic biology; Riboswitch; Aptamer; Ribozyme; Aptazyme; HIGH-THROUGHPUT SELECTION; IN-VIVO EVOLUTION; GENE-EXPRESSION; CONFORMATIONAL CAPTURE; MECHANISTIC INSIGHTS; RATIONAL DESIGN; BINDING; APTAMER; RIBOZYME; TRANSLATION;

D O I：

10.1016/j.febslet.2012.02.038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Riboswitches are natural RNA-based genetic switches that sense small-molecule metabolites and regulate in response the expression of the corresponding metabolic genes. Within the last years, several engineered riboswitches have been developed that act on various stages of gene expression. These switches can be engineered to respond to any ligand of choice and are therefore of great interest for synthetic biology. In this review, we present an overview of engineered riboswitches and discuss their application in conditional gene expression systems. We will provide structural and mechanistic insights and point out problems and recent trends in the development of engineered riboswitches. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2076 / 2083

页数：8

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