Rapamycin inhibits FBXW7 loss-induced epithelial-mesenchymal transition and cancer stem cell-like characteristics in colorectal cancer cells

被引：63

作者：

Wang, Yuli ^{[1
,2
]}

Liu, Yueyong ^{[3
]}

Lu, Jing ^{[1
,2
]}

Zhang, Pengju ^{[3
,4
]}

Wang, Yunshan ^{[1
,2
]}

Xu, Yangyang ^{[1
,2
]}

Wang, Zeran ^{[3
]}

Mao, Jian-Hua ^{[3
]}

Wei, Guangwei ^{[1
,2
,3
]}

机构：

[1] Shandong Univ, Sch Med, Dept Anat, Minist Educ, Jinan 250012, Shandong, Peoples R China

[2] Shandong Univ, Sch Med, Minist Educ, Key Lab Expt Teratol, Jinan 250012, Shandong, Peoples R China

[3] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA

[4] Shandong Univ, Sch Med, Dept Biochem & Mol Biol, Jinan 250012, Shandong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 434卷 / 02期

基金：

美国国家卫生研究院; 中国国家自然科学基金;

关键词：

Fbxw7; Epithelial-mesenchymal transition; Cell migration and invasion; Cancer stem cell; Colorectal cancer; TUMOR-SUPPRESSOR; UBIQUITIN LIGASE; AURORA-A; FBW7; DEGRADATION; METASTASIS; MTOR; PTEN; MICE;

D O I：

10.1016/j.bbrc.2013.03.077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Increased cell migration and invasion lead to cancer metastasis and are crucial to cancer prognosis. In this study, we explore whether FBXW7 plays any role in metastatic process. We show that depletion of FBXW7 induces epithelial-mesenchymal transition (EMT) in human colon cancer cells along with the increase in cell migration and invasion. Moreover, FBXW7 deficiency promotes the generation of colon cancer stem-like cells in tumor-sphere culture. mTOR inhibition by rapamycin suppresses FBXW7 loss-driven EMT, invasion and stemness. Our results define the FBXW7/mTOR axis as a novel EMT pathway that mediates cancer invasion. Published by Elsevier Inc.

引用

页码：352 / 356

页数：5

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