A paramagnetic microspheres based automation-friendly rapid chemiluminescent immunoassay method for sensitive detection of chicken interferon-γ

被引:13
作者
Dai, Hua [1 ]
Zhu, Jing [1 ]
Yang, Zhanjun [1 ]
Li, Juan [1 ]
Jiao, Xin'an [1 ]
Hu, Xiaoya [1 ]
Wang, Joseph [2 ]
机构
[1] Yangzhou Univ, Jiangsu Key Lab Zoonosis, Sch Med, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China
[2] Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 USA
基金
中国国家自然科学基金;
关键词
RESOLUTION STRATEGY; ENZYME-IMMUNOASSAY; MITOGEN; ANTIBODIES; RESPONSES; ANTIGEN; SYSTEM; ALPHA;
D O I
10.1039/c3cc38040h
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We describe an automation-friendly chemiluminescent immunoassay method for sensitive and rapid determination of chicken interferon-gamma at the sub-picogram per millilitre level based on the monoclonal antibody immobilized paramagnetic microspheres and flow-through system.
引用
收藏
页码:1708 / 1710
页数:3
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