Differential Transcript Accumulation of Dhydrin and Beta-glucosidase Genes to Cold-induced Oxidative Stress in Chickpea

In this study, accumulation of H2O2, malondialdehyde (MDA) (as cold-induced oxidative stress indicators), the transcript levels of dehydrin and beta-glucosidase genes (involved in metabolic responses) was evaluated in chickpea cv. Jam, using qRT-PCR during control, cold acclimation (CA), cold stress (CS), recovery, and freezing phases. Results showed the existence of wide range of genetic capacity in the cultivar to increase cold tolerance when environmental conditions change. Significant increase in H2O2 and MDA content during CA phase indicated that seedlings perceived cold signaling that resulted in remarkable increase in the transcript levels of dehydrin and beta-glucosidase genes as part of defense responses of plants. Balancing the expression of these genes and oxidative stress indicators showed the interplay between two major defense and injury pathways. During freezing phase, the higher transcript levels of these genes in acclimated plants compared to non-acclimated plants showed a more active role for plant cells. An incapability of defense machine in non-acclimated plants was a limiting factor determining the low potential of chickpea plants to freezing phase. It was suggested that adjustment and metabolic alterations like dehydrin and beta-glucosidase genes, especially after CA phase and, thereby, decrease in oxidative stress indicators, could be a reason for relative cold tolerance in chickpea.