Molecular basis of resistance to tribenuron-methyl in Descurainia Sophia (L.) populations from China

被引:26
作者
Han, Xue Jing [1 ]
Dong, Yi [1 ]
Sun, Xiao Na [1 ]
Li, Xue Feng [1 ]
Zheng, Ming Qi [1 ]
机构
[1] China Agr Univ, Dept Appl Chem, Beijing 100193, Peoples R China
关键词
AHAS enzyme; AHAS gene; Flixweed; Herbicide resistance; ALS-INHIBITING HERBICIDES; POPPY PAPAVER-RHOEAS; ACETOLACTATE-SYNTHASE; LACTUCA-SERRIOLA; WHEAT-BELT; MUTATIONS; MECHANISMS; EVOLUTION;
D O I
10.1016/j.pestbp.2012.07.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two Descurainia Sophia populations, HB16 and HB08, were discovered in China and exhibit high resistance levels to tribenuron-methyl. Resistance ratio values of HB16 and HB08 were 153.47 and 651.20, respectively. The extractable acetohydroxy acid synthase (AHAS) activity was similar between resistant and susceptible populations. However, AHAS from HB16 and HB08 was less sensitive to inhibition of tribenuron-methyl comparing to susceptible Population. The tribenuron-methyl I-50 values for HB16 and HB08 were 46.25 and 54.07 greater than that for susceptible population respectively. Pro-197-Ser and Pro-197-Leu mutations were identified in AHAS extracted from HB16 and HB08 plants respectively. AHAS insensitivity of resistant D. Sophia caused by Pro197 mutation could be responsible for high resistance to tribenuron-methyl. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:77 / 81
页数:5
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