Metagenomics of human microbiome: beyond 16s rDNA

被引:40
作者
Gosalbes, M. J. [1 ,2 ]
Abellan, J. J. [1 ,2 ]
Durban, A. [1 ,2 ]
Perez-Cobas, A. E. [1 ,2 ]
Latorre, A. [1 ,2 ]
Moya, A. [1 ,2 ]
机构
[1] Univ Valencia, Cavanilles Inst Biodivers & Evolutionary Biol, Ctr Publ Hlth Res CSISP, Joint Res Unit Genom & Hlth, Valencia 46020, Spain
[2] CIBEResp, Madrid, Spain
关键词
Epigenetic landscape; evolutionary development; intestinal microbiota; metabolic functions; metatranscriptomics;
D O I
10.1111/j.1469-0691.2012.03865.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Clin Microbiol Infect 2012; 18 (Suppl. 4): 4749 Abstract The gut microbiota presents a symbiotic relationship with the human host playing a beneficial role in human health. Since its establishment, the bacterial community is subjected to the influence of many different factors that shape its composition within each individual. However, an important convergence is observed at functional level in the gut microbiota. A metatranscriptomic study of healthy individuals showed homogeneity in the composition of the active microbiota that increased further at functional level.
引用
收藏
页码:47 / 49
页数:3
相关论文
共 13 条
[1]   Metatranscriptome Analysis of the Human Fecal Microbiota Reveals Subject-Specific Expression Profiles, with Genes Encoding Proteins Involved in Carbohydrate Metabolism Being Dominantly Expressed [J].
Booijink, Carien C. G. M. ;
Boekhorst, Jos ;
Zoetendal, Erwin G. ;
Smidt, Hauke ;
Kleerebezem, Michiel ;
de Vos, Willem M. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (16) :5533-5540
[2]   The Ribosomal Database Project: improved alignments and new tools for rRNA analysis [J].
Cole, J. R. ;
Wang, Q. ;
Cardenas, E. ;
Fish, J. ;
Chai, B. ;
Farris, R. J. ;
Kulam-Syed-Mohideen, A. S. ;
McGarrell, D. M. ;
Marsh, T. ;
Garrity, G. M. ;
Tiedje, J. M. .
NUCLEIC ACIDS RESEARCH, 2009, 37 :D141-D145
[3]   Microbial Prevalence, Diversity and Abundance in Amniotic Fluid During Preterm Labor: A Molecular and Culture-Based Investigation [J].
DiGiulio, Daniel B. ;
Romero, Roberto ;
Amogan, Harold P. ;
Kusanovic, Juan Pedro ;
Bik, Elisabeth M. ;
Gotsch, Francesca ;
Kim, Chong Jai ;
Erez, Offer ;
Edwin, Sam ;
Relman, David A. .
PLOS ONE, 2008, 3 (08)
[4]   Gut flora in health and disease [J].
Guarner, F ;
Malagelada, JR .
LANCET, 2003, 361 (9356) :512-519
[5]  
Hall BK., 1999, EVOLUTIONARY DEV BIO
[6]   Metatranscriptomic Approach to Analyze the Functional Human Gut Microbiota [J].
Jose Gosalbes, Maria ;
Durban, Ana ;
Pignatelli, Miguel ;
Jose Abellan, Juan ;
Jimenez-Hernandez, Nuria ;
Elena Perez-Cobas, Ana ;
Latorre, Amparo ;
Moya, Andres .
PLOS ONE, 2011, 6 (03)
[7]   ON PHENOCOPIES, THEIR DEVELOPMENTAL PHYSIOLOGY AND GENETIC MEANING [J].
LANDAUER, W .
AMERICAN NATURALIST, 1958, 92 (865) :201-213
[8]   Microbes in Gastrointestinal Health and Disease [J].
Neish, Andrew S. .
GASTROENTEROLOGY, 2009, 136 (01) :65-80
[9]   A human gut microbial gene catalogue established by metagenomic sequencing [J].
Qin, Junjie ;
Li, Ruiqiang ;
Raes, Jeroen ;
Arumugam, Manimozhiyan ;
Burgdorf, Kristoffer Solvsten ;
Manichanh, Chaysavanh ;
Nielsen, Trine ;
Pons, Nicolas ;
Levenez, Florence ;
Yamada, Takuji ;
Mende, Daniel R. ;
Li, Junhua ;
Xu, Junming ;
Li, Shaochuan ;
Li, Dongfang ;
Cao, Jianjun ;
Wang, Bo ;
Liang, Huiqing ;
Zheng, Huisong ;
Xie, Yinlong ;
Tap, Julien ;
Lepage, Patricia ;
Bertalan, Marcelo ;
Batto, Jean-Michel ;
Hansen, Torben ;
Le Paslier, Denis ;
Linneberg, Allan ;
Nielsen, H. Bjorn ;
Pelletier, Eric ;
Renault, Pierre ;
Sicheritz-Ponten, Thomas ;
Turner, Keith ;
Zhu, Hongmei ;
Yu, Chang ;
Li, Shengting ;
Jian, Min ;
Zhou, Yan ;
Li, Yingrui ;
Zhang, Xiuqing ;
Li, Songgang ;
Qin, Nan ;
Yang, Huanming ;
Wang, Jian ;
Brunak, Soren ;
Dore, Joel ;
Guarner, Francisco ;
Kristiansen, Karsten ;
Pedersen, Oluf ;
Parkhill, Julian ;
Weissenbach, Jean .
NATURE, 2010, 464 (7285) :59-U70
[10]   Organismal, genetic, and transcriptional variation in the deeply sequenced gut microbiomes of identical twins [J].
Turnbaugh, Peter J. ;
Quince, Christopher ;
Faith, Jeremiah J. ;
McHardy, Alice C. ;
Yatsunenko, Tanya ;
Niazi, Faheem ;
Affourtit, Jason ;
Egholm, Michael ;
Henrissat, Bernard ;
Knight, Rob ;
Gordon, Jeffrey I. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (16) :7503-7508