Application of low-energy scanning transmission electron microscopy for the study of Pt-nanoparticle uptake in human colon carcinoma cells

被引：12

作者：

Blank, Holger ^{[1
]}

Schneider, Reinhard ^{[1
]}

Gerthsen, Dagmar ^{[1
]}

Gehrke, Helge ^{[2
]}

Jarolim, Katharina ^{[2
]}

Marko, Doris ^{[2
]}

机构：

[1] Karlsruher Inst Technol KIT, Lab Elektronenmikroskopie, D-76128 Karlsruhe, Germany

[2] Univ Vienna, Dept Food Chem & Toxicol, A-1090 Vienna, Austria

来源：

NANOTOXICOLOGY | 2014年 / 8卷 / 04期

关键词：

electron microscopy; low-energy HAADF STEM; nanoparticles; cells; quantification of cellular dose; IN-VITRO; DOSIMETRY; TOXICITY; DAMAGE;

D O I：

10.3109/17435390.2013.796535

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

High-angle annular dark-field scanning transmission electron microscopy (HAADF STEM) in a scanning electron microscope facilitates the acquisition of images with high chemical sensitivity and high resolution. HAADF STEM at low electron energies is particularly suited to image nanoparticles (NPs) in thin cell sections which are not subjected to poststaining procedures as demonstrated by comparison with bright-field TEM. High membrane contrast is achieved and distinction of NPs with different chemical composition is possible at first sight. Low-energy HAADF STEM was applied to systematically study the uptake of Pt-NPs with a broad size distribution in HT29 colon carcinoma cells as a function of incubation time and incubation temperature. The cellular dose was quantified, that is, the amount and number density of NPs taken up by the cells, as well as the particle-size distribution. The results show a strong dependence of the amount of incubated NPs on the exposure time which can be understood by considering size-dependent diffusion and gravitational settling of the NPs in the cell culture medium.

引用

页码：433 / 446

页数：14

共 40 条

[11]

Frank J., 2006, Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell

[12]

Gehrke H., 2012, NANOTOXICOLOGY

[13] Platinum nanoparticles and their cellular uptake and DNA platination at non-cytotoxic concentrations [J].

Gehrke, Helge ;

Pelka, Joanna ;

Hartinger, Christian G. ;

Blank, Holger ;

Bleimund, Felix ;

Schneider, Reinhard ;

Gerthsen, Dagmar ;

Braese, Stefan ;

Crone, Marlene ;

Tuerk, Michael ;

Marko, Doris .

ARCHIVES OF TOXICOLOGY, 2011, 85 (07) :799-812

[14] Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells [J].

Gerloff, Kirsten ;

Albrecht, Catrin ;

Boots, Agnes W. ;

Foerster, Irmgard ;

Schins, Roel P. F. .

NANOTOXICOLOGY, 2009, 3 (04) :355-364

[15]

Heinrich K.F. J., 1981, ELECT BEAM XRAY MICR

[16] Far-field optical nanoscopy [J].

Hell, Stefan W. .

SCIENCE, 2007, 316 (5828) :1153-1158

[17] ISDD: A computational model of particle sedimentation, diffusion and target cell dosimetry for in vitro toxicity studies [J].

Hinderliter, Paul M. ;

Minard, Kevin R. ;

Orr, Galya ;

Chrisler, William B. ;

Thrall, Brian D. ;

Pounds, Joel G. ;

Teeguarden, Justin G. .

PARTICLE AND FIBRE TOXICOLOGY, 2010, 7

[18] STEM mode in the SEM: A practical tool for nanotoxicology [J].

Hondow, Nicole ;

Harrington, John ;

Brydson, Rik ;

Doak, Shareen H. ;

Singh, Neenu ;

Manshian, Bella ;

Brown, Andy .

NANOTOXICOLOGY, 2011, 5 (02) :215-227

[19] Evaluation of cellular influences of platinum nanoparticles by stable medium dispersion [J].

Horie, Masanori ;

Kato, Haruhisa ;

Endoh, Shigehisa ;

Fujita, Katsuhide ;

Nishio, Keiko ;

Komaba, Lilian Kaede ;

Fukui, Hiroko ;

Nakamura, Ayako ;

Miyauchi, Arisa ;

Nakazato, Tetsuya ;

Kinugasa, Shinichi ;

Yoshida, Yasukazu ;

Hagihara, Yoshihisa ;

Morimoto, Yasuo ;

Iwahashi, Hitoshi .

METALLOMICS, 2011, 3 (11) :1244-1252

[20] AN EMPIRICAL STOPPING POWER RELATIONSHIP FOR LOW-ENERGY ELECTRONS [J].

JOY, DC ;

LUO, S .

SCANNING, 1989, 11 (04) :176-180

← 1 2 3 4 →