The novel amyloid-beta peptide aptamer inhibits intracellular amyloid-beta peptide toxicity

被引：6

作者：

Wang, Xu ^{[1
]}

Yang, Yi ^{[1
]}

Jia, Mingyue ^{[1
]}

Ma, Chi ^{[2
]}

Wang, Mingyu ^{[1
]}

Che, Lihe ^{[3
]}

Yang, Yu ^{[1
]}

Wu, Jiang ^{[1
]}

机构：

[1] Jilin Univ, Hosp 1, Dept Neurol, Changchun 130021, Jilin Province, Peoples R China

[2] Jilin Univ, Hosp 1, Dept Neurosurg, Changchun 130021, Jilin Province, Peoples R China

[3] Jilin Univ, Hosp 1, Dept Infect, Changchun 130021, Jilin Province, Peoples R China

来源：

NEURAL REGENERATION RESEARCH | 2013年 / 8卷 / 01期

基金：

中国国家自然科学基金;

关键词：

neural regeneration; neurodegenerative disease; gene therapy; Alzheimer's disease; aptamer; amyloid beta peptide; amyloid beta peptide binding alcohol dehydrogenase; decoy peptide; thioredoxin; mitochondrial dysfunction; molecular cloning; grants-supported paper; photographs-containing paper; neuroregeneration; INDUCED MITOCHONDRIAL DYSFUNCTION; ALZHEIMERS-DISEASE; A-BETA; MOUSE MODEL; NEURODEGENERATIVE DISEASES; OXIDATIVE STRESS; TRANSGENIC MICE; ACCUMULATION; ABAD; PROTEIN;

D O I：

10.3969/j.issn.1673-5374.2013.01.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Amyloid beta peptide binding alcohol dehydrogenase (ABAD) decoy peptide (DP) can competitively antagonize binding of amyloid beta peptide to ABAD and inhibit the cytotoxic effects of amyloid beta peptide. Based on peptide aptamers, the present study inserted ABAD-DP into the disulfide bond of human thioredoxin (TRX) using molecular cloning technique to construct a fusion gene that can express the TRX1-ABAD-DP-TRX2 aptamer. Moreover, adeno-associated virus was used to allow its stable expression. Immunofluorescent staining revealed the co-expression of the transduced fusion gene TRX1-ABAD-DP-TRX2 and amyloid beta peptide in NIH-3T3 cells, indicating that the TRX1-ABAD-DP-TRX2 aptamer can bind amyloid beta peptide within cells. In addition, cell morphology and MU results suggested that TRX1-ABAD-DP-TRX2 attenuated amyloid beta peptide-induced SH-SY5Y cell injury and improved cell viability. These findings confirmed the possibility of constructing TRX-based peptide aptamer using ABAD-DP. Moreover, TRX1-ABAD-DP-TRX2 inhibited the cytotoxic effect of amyloid beta peptide.

引用

页码：39 / 48

页数：10

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