Quantitative Microbial Risk Assessment and Molecular Biology: Paths to Integration

被引：40

作者：

Haas, Charles N. ^{[1
]}

机构：

[1] Drexel Univ, Dept Civil Architectural & Environm Engn, Philadelphia, PA 19104 USA

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2020年 / 54卷 / 14期

关键词：

REAL-TIME PCR; DOSE-RESPONSE; TAXONOMIC CHARACTERIZATION; HARVESTED RAINWATER; WATER; BACTERIAL; SURVIVAL; COMMUNITIES; LEGIONELLA; CRYPTOSPORIDIUM;

D O I：

10.1021/acs.est.0c00664

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Quantitative microbial risk assessment (QMRA) has now been in use for over 35 years and has formed the basis for developing criteria for ensuring public health related to water, food, and remediation, to name a few areas. The initial data for QMRA (both in exposure assessment and in dose response assessment) came from measurements using assays for viability, such as plate counts, plaque assays, or animal infectivity. With the increasing use of molecular methods for the measurement of microorganisms in the environment, it has become important to assess how to use such data to estimate infectious disease risks. The limitations to the use of such data and needs to resolve the limitations will be addressed.

引用

页码：8539 / 8546

页数：8

共 75 条

[1] Amplicon-based taxonomic characterization of bacteria in urban and peri-urban roof-harvested rainwater stored in tanks [J].

Ahmed, W. ;

Staley, C. ;

Hamilton, K. A. ;

Beale, D. J. ;

Sadowsky, M. J. ;

Toze, S. ;

Haas, C. N. .

SCIENCE OF THE TOTAL ENVIRONMENT, 2017, 576 :326-334

[2] Bioinformatics tools to assess metagenomic data for applied microbiology [J].

Almeida, Otavio G. G. ;

De Martinis, Elaine C. P. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 103 (01) :69-82

[3]

[Anonymous], RISK ASS FED GOV MAN

[4]

Bartlett J. M. S., 2003, PCR PROTOCOLS METHOD, V226, P1

[5] Metagenomics and the development of viral water quality tools [J].

Bibby, Kyle ;

Crank, Katherine ;

Greaves, Justin ;

Li, Xiang ;

Wu, Zhenyu ;

Hamza, Ibrahim A. ;

Stachler, Elyse .

NPJ CLEAN WATER, 2019, 2 (1)

[6] Script and identity - the politics of writing in South Asia: an introduction [J].

Brandt, Carmen ;

Sohoni, Pushkar .

SOUTH ASIAN HISTORY AND CULTURE, 2018, 9 (01) :1-15

[7] The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies [J].

Brooks, J. Paul ;

Edwards, David J. ;

Harwich, Michael D., Jr. ;

Rivera, Maria C. ;

Fettweis, Jennifer M. ;

Serrano, Myrna G. ;

Reris, Robert A. ;

Sheth, Nihar U. ;

Huang, Bernice ;

Girerd, Philippe ;

Strauss, Jerome F., III ;

Jefferson, Kimberly K. ;

Buck, Gregory A. .

BMC MICROBIOLOGY, 2015, 15

[8] Dead or Alive: Molecular Assessment of Microbial Viability [J].

Cangelosi, Gerard A. ;

Meschke, John S. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014, 80 (19) :5884-5891

[9] Prospects for applying virulence factor-activity relationships (VFAR) to emerging waterborne pathogens [J].

Cangelosi, Gerard A. .

JOURNAL OF WATER AND HEALTH, 2009, 7 :S64-S74

[10] Survival and recovery of seeded HIV in water and wastewater [J].

Casson, LW ;

Ritter, MOD ;

Cossentino, LM ;

Gupta, P .

WATER ENVIRONMENT RESEARCH, 1997, 69 (02) :174-179

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