Matricellular protein SPARCL1 regulates tumor microenvironment-dependent endothelial cell heterogeneity in colorectal carcinoma

被引：71

作者：

Naschberger, Elisabeth ^{[1
]}

Liebl, Andrea ^{[1
]}

Schellerer, Vera S. ^{[2
]}

Schuetz, Manuela ^{[1
]}

Britzen-Laurent, Nathalie ^{[1
]}

Koelbel, Patrick ^{[1
]}

Schaal, Ute ^{[1
]}

Haep, Lisa ^{[1
]}

Regensburger, Daniela ^{[1
]}

Wittmann, Thomas ^{[1
]}

Klein-Hitpass, Ludger ^{[3
]}

Rau, Tilman T. ^{[4
]}

Dietel, Barbara ^{[5
]}

Meniel, Valerie S. ^{[6
]}

Clarke, Alan R. ^{[6
]}

Merkel, Susanne ^{[2
]}

Croner, Roland S. ^{[2
]}

Hohenberger, Werner ^{[2
]}

Stuerzl, Michael ^{[1
]}

机构：

[1] Friedrich Alexander Univ Erlangen Nuremberg, Translat Res Ctr, Univ Med Ctr Erlangen, Div Mol & Expt Surg, Erlangen, Germany

[2] Friedrich Alexander Univ Erlangen Nuremberg, Univ Med Ctr Erlangen, Dept Surg, Erlangen, Germany

[3] Univ Med Ctr Essen, Fac Med, Inst Cell Biol, Essen, Germany

[4] Friedrich Alexander Univ Erlangen Nuremberg, Univ Med Ctr Erlangen, Inst Pathol, Erlangen, Germany

[5] Friedrich Alexander Univ Erlangen Nuremberg, Univ Med Ctr Erlangen, Translat Res Ctr, Dept Cardiol & Angiol, Erlangen, Germany

[6] Cardiff Univ, European Canc Stem Cell Res Inst, Cardiff, S Glam, Wales

来源：

JOURNAL OF CLINICAL INVESTIGATION | 2016年 / 126卷 / 11期

关键词：

GUANYLATE-BINDING PROTEIN-1; SECRETED-PROTEIN; GROWTH-FACTOR; NOTCH TARGET; EXPRESSION; ANGIOGENESIS; CANCER; SURVIVAL; PROGRESSION; ACTIVATION;

D O I：

10.1172/JCI78260

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Different tumor microenvironments (TMEs) induce stromal cell plasticity that affects tumorigenesis. The impact of TME-dependent heterogeneity of tumor endothelial cells (TECs) on tumorigenesis is unclear. Here, we isolated pure TECs from human colorectal carcinomas (CRCs) that exhibited TMEs with either improved (Th1-TME CRCs) or worse clinical prognosis (control-TME CRCs). Transcriptome analyses identified markedly different gene clusters that reflected the tumorigenic and angiogenic activities of the respective TMEs. The gene encoding the matricellular protein SPARCL1 was most strongly upregulated in Th1-TME TECs. It was also highly expressed in ECs in healthy colon tissues and Th1-TME CRCs but low in control TME CRCs. In vitro, SPARCL1 expression was induced in confluent, quiescent ECs and functionally contributed to EC quiescence by inhibiting proliferation, migration, and sprouting, whereas siRNA-mediated knockdown increased sprouting. In human CRC tissues and mouse models, vessels with SPARCL1 expression were larger and more densely covered by mural cells. SPARCL1 secretion from quiescent ECs inhibited mural cell migration, which likely led to stabilized mural cell coverage of mature vessels. Together, these findings demonstrate TME-dependent intertumoral TEC heterogeneity in CRC. They further indicate that TEC heterogeneity is regulated by SPARCL1, which promotes the cell quiescence and vessel homeostasis contributing to the favorable prognoses associated with Th1-TME CRCs.

引用

页码：4187 / 4204

页数：18