General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus

被引：11

作者：

Costal-Oliveira, F. ^{[1
,2
]}

Duarte, C. G. ^{[1
,2
]}

Machado de Avila, R. A. ^{[1
,2
]}

Melo, M. M. ^{[4
]}

Bordon, K. C. F. ^{[5
]}

Arantes, E. C. ^{[5
]}

Paredes, N. C. ^{[6
]}

Tintaya, B. ^{[6
]}

Bonilla, C. ^{[6
]}

Bonilla, R. E. ^{[1
,2
]}

Suarez, W. S. ^{[6
]}

Yarleque, A. ^{[7
]}

Fernandez, J. M. ^{[7
]}

Kalapothakis, E.

Chavez-Olortegui, Carlos ^{[1
,2
,3
]}

机构：

[1] Univ Fed Minas Gerais, Dept Bioquim, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Dept Imunol, BR-31270901 Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, Brazil

[4] Univ Fed Minas Gerais, Escola Vet, BR-31270901 Belo Horizonte, MG, Brazil

[5] Univ Sao Paulo, Dept Quim & Fis, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP, Brazil

[6] Inst Nacl Salud, Lima, Peru

[7] Univ Nacl Mayor San Marcos, Lima 14, Peru

来源：

TOXICON | 2012年 / 60卷 / 05期

关键词：

Hadruroides lunatus scorpion venom; Phospholipasic activity; LD50; Anti-H. lunatus sera; MASS-SPECTROMETRY ANALYSIS; POLYACRYLAMIDE-GELS; PROTEINS; CHANNELS; TOXINS; SEQUENCE; ENZYMES; FAMILY;

D O I：

10.1016/j.toxicon.2012.06.013

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

This communication describes the general biochemical properties and some immunological characteristics of the venom from the Peruvian scorpion Hadruroides lunatus, which is the most medically relevant species in Peru. The soluble venom of this scorpion is toxic to mice, the LD50 determined was 0.1 mg/kg and 21.55 mg/kg when the venom was injected intracranial or intraperitoneally, respectively. The soluble venom displayed proteolytic, hyaluronidasic, phospholipasic and cardiotoxic activities. High performance liquid chromatography of the soluble venom resulted in the separation of 20 fractions. Two peptides with phospholipasic activity were isolated to homogeneity and their molecular masses determined by mass spectrometry (MALDI TOF). Anti-H. lunatus venom sera were produced in rabbits. Western blotting analysis showed that most of the protein content of this venom is immunogenic. H. lunatus anti-venom displayed consistent cross-reactivity with venom antigens from the new World-scorpions Tityus serrulatus and Centruroides sculpturatus venoms; however, a weaker reactivity was observed against the venom antigens from the old World-scorpion Androctonus australis Hector. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：934 / 942

页数：9

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