QCM-Based DNA Biosensor for Salmonella typhimurium Detection

被引：0

作者：

Waiwijit, Uraiwan ^{[1
]}

Jaruwongrungsee, Kata ^{[1
]}

Chokesajjawatee, Nipa ^{[2
]}

Promjai, Jurairat ^{[1
]}

Lomas, Tanom ^{[1
]}

Sritongkham, Pornpimol ^{[3
]}

Tuantranont, Adisorn ^{[1
]}

机构：

[1] Natl Elect & Comp Technol Ctr NECTEC, Nanoelect & MEMS Lab, Pathum Thani, Thailand

[2] Natl Ctr Genet Engn & Biotechnol BIOTEC, Food Biotechnol Lab, Pathum Thani, Thailand

[3] Mahidol Univ, Fac Engn, Dept Biomed Engn, Nakhon Pathom, Thailand

来源：

2012 IEEE INTERNATIONAL CONFERENCE ON ELECTRON DEVICES AND SOLID STATE CIRCUIT (EDSSC) | 2012年

关键词：

QCM; DNA biosensor; Salmonella typhimurium;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

This work demonstrated a QCM biosensor for the detection of Salmonella typhimurium using the specific DNA probe. A thiol DNA probe was immobilized onto quartz surface coated with gold through self-assembly via Au-S bonding formation. 6-mercapto-1-hexanol (MCH) was used as blocking reagent against non-specific DNA binding. Target DNA with 94 bp (single strand) was hybridized with its probe resulted in a decrease in the resonance frequency of the QCM biosensor. It was demonstrated that the QCM biosensor could recognize the target DNA sequence within 40-70 min. The frequency shift signal was distinguishable along with the increase of target concentration from 0.1 to 1.0 mu M.

引用

页数：3

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