Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration

被引：21

作者：

Esteca, Marcos, V ^{[1
]}

Severino, Matheus B. ^{[1
]}

Silvestre, Joao G. ^{[2
]}

dos Santos, Gustavo Palmeira ^{[1
]}

Tamborlin, Leticia ^{[3
,4
]}

Luchessi, Augusto D. ^{[3
,4
]}

Moriscot, Anselmo S. ^{[2
]}

Gustafsson, Asa B. ^{[5
]}

Baptista, Igor L. ^{[1
,6
]}

机构：

[1] Univ Estadual Campinas, Sch Appl Sci, Lab Cell & Tissue Biol, BR-13484350 Limeira, Brazil

[2] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508900 Sao Paulo, Brazil

[3] Univ Estadual Campinas, Sch Appl Sci, Lab Biotechnol, BR-13484350 Limeira, Brazil

[4] Sao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, Brazil

[5] Univ Calif San Diego, Skaggs Sch Pharm & Pharmacol Sci, La Jolla, CA 92093 USA

[6] Univ Estadual Campinas, Sch Appl Sci, Rua Pedro Zaccaria 1300, BR-13484350 Limeira, Brazil

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2020年 / 21卷 / 21期

基金：

巴西圣保罗研究基金会;

关键词：

satellite cells; differentiation; mitochondria; mitophagy; MOUSE MODEL; MITOCHONDRIAL; MITOPHAGY; INJURY; DEFICITS; FATIGUE; PAX7;

D O I：

10.3390/ijms21218007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The high capacity of the skeletal muscle to regenerate is due to the presence of muscle stem cells (MuSCs, or satellite cells). The E3 ubiquitin ligase Parkin is a key regulator of mitophagy and is recruited to mitochondria during differentiation of mouse myoblast cell line. However, the function of mitophagy during regeneration has not been investigated in vivo. Here, we have utilized Parkin deficient (Parkin(-/-)) mice to investigate the role of Parkin in skeletal muscle regeneration. We found a persistent deficiency in skeletal muscle regeneration in Parkin(-/-) mice after cardiotoxin (CTX) injury with increased area of fibrosis and decreased cross-sectional area (CSA) of myofibres post-injury. There was also a significant modulation of MuSCs differentiation and mitophagic markers, with altered mitochondrial proteins during skeletal muscle regeneration in Parkin(-/-) mice. Our data suggest that Parkin-mediated mitophagy plays a key role in skeletal muscle regeneration and is necessary for MuSCs differentiation.

引用

页码：1 / 19

页数：19

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