Heterogeneity of miR-10b expression in circulating tumor cells

被引：37

作者：

Gasch, Christin ^{[1
]}

Plummer, Prue N. ^{[1
]}

Jovanovic, Lidija ^{[2
,3
,4
]}

McInnes, Linda M. ^{[5
]}

Wescott, David ^{[1
]}

Saunders, Christobel M. ^{[5
]}

Schneeweiss, Andreas ^{[6
,7
]}

Wallwiener, Markus ^{[6
,7
]}

Nelson, Colleen ^{[2
,3
,4
]}

Spring, Kevin J. ^{[8
,9
]}

Riethdorf, Sabine ^{[10
]}

Thompson, Erik W. ^{[3
,4
,11
,12
]}

Pantel, Klaus ^{[10
]}

Mellick, Albert S. ^{[1
,13
]}

机构：

[1] Deakin Univ, Sch Med, Geelong, Vic 3217, Australia

[2] Queensland Univ Technol, Australian Prostate Canc Res Ctr Queensland, Brisbane, Qld 4001, Australia

[3] Queensland Univ Technol, Inst Hlth & Biomed Innovat, Brisbane, Qld 4001, Australia

[4] Queensland Univ Technol, Sch Biomed Sci, Brisbane, Qld 4001, Australia

[5] Univ Western Australia, Sch Surg, Perth, WA 6009, Australia

[6] Natl Ctr Tumor Dis, Heidelberg, Germany

[7] Heidelberg Univ, Dept Obstet & Gynecol, Heidelberg, Germany

[8] Liverpool Hosp, Ingham Inst Appl Med Res, Liverpool, NSW, Australia

[9] Univ Western Sydney, Sch Med, Liverpool Clin Sch, Penrith, NSW 1797, Australia

[10] Univ Med Ctr Hamburg Eppendorf, Dept Tumor Biol, Hamburg, Germany

[11] Univ Melbourne, St Vincents Inst Med Res, Melbourne, Vic, Australia

[12] Univ Melbourne, Dept Surg, Melbourne, Vic, Australia

[13] Univ New S Wales, Sch Med, Sydney, NSW 2052, Australia

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

英国医学研究理事会; 澳大利亚国家健康与医学研究理事会;

关键词：

IN-SITU HYBRIDIZATION; METASTATIC BREAST-CANCER; PERIPHERAL-BLOOD; MICRORNA EXPRESSION; MESSENGER-RNA; MUTATIONS; INVASION; MIRNAS;

D O I：

10.1038/srep15980

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Circulating tumor cells (CTCs) in the blood of cancer patients are recognized as important potential targets for future anticancer therapies. As mediators of metastatic spread, CTCs are also promising to be used as `liquid biopsy' to aid clinical decision-making. Recent work has revealed potentially important genotypic and phenotypic heterogeneity within CTC populations, even within the same patient. MicroRNAs (miRNAs) are key regulators of gene expression and have emerged as potentially important diagnostic markers and targets for anti-cancer therapy. Here, we describe a robust in situ hybridization (ISH) protocol, incorporating the CellSearch (R) CTC detection system, enabling clinical investigation of important miRNAs, such as miR-10b on a cell by cell basis. We also use this method to demonstrate heterogeneity of such as miR-10b on a cell-by-cell basis. We also use this method to demonstrate heterogeneity of miR-10b in individual CTCs from breast, prostate and colorectal cancer patients.

引用

页数：10

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