Disodium Salt of 2-(5-phenyl-1, 3, 4-oxadiazol-2-yl) Phenyl Phosphate as a Fluorescent Probe for Determination of ALP

被引：2
作者：
Lei Yingjie [1 ]
Ouyang Jie [1 ]
Ding Mei [1 ]
机构：
[1] Tianjin Univ Technol, Sch Chem & Chem Engn, Tianjin, Peoples R China
来源：
APPLIED MECHANICS AND MECHANICAL ENGINEERING II, PTS 1 AND 2 | 2012年 / 138-139卷
关键词：
ALP; fluorescent probe; 2-(5-phenyl-1,3,4-oxadiazol-2-yl)phenol; synthesis; FLUOROMETRIC-DETERMINATION; HUMAN SERUM; ALKALINE; SUBSTRATE;
D O I：
10.4028/www.scientific.net/AMM.138-139.1109
中图分类号：
TH [机械、仪表工业];
学科分类号：
0802 ;
摘要：
A convenient method to synthesize disodium salt of 2-(5-phenyl-1, 3, 4-oxadiazol-2-yl) phenyl phosphate (OXDP), a substrate for the fluorescent determination of alkaline phosphatase (ALP) activity, via an one-pot procedure of condensation between the benzoic acid and 2-hydroxybenzhydrazide at triethylamine alkaline solution in the presence of propylphosphonic anhydride, was realized. Preliminary tests reveal that the lessened fluorescence intensity resulting from enzyme reaction is proportional to ALP activity in the range of 1.0-25.0 U/L with the detection limit of 0.25U/L, which can be successfully applied to assess ALP in human serum with good precision.
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收藏
页码：1109 / 1114
页数：6
相关论文
共 15 条
  • [1] The effect of alkaline phosphatase inhibitors on intracellular lipid accumulation in preadipocytes isolated from human mammary tissue
    Ali, Aus T.
    Penny, Clem B.
    Paiker, Janice E.
    Psaras, George
    Ikram, Faisel
    Crowther, Nigel J.
    [J]. ANNALS OF CLINICAL BIOCHEMISTRY, 2006, 43 : 207 - 213
  • [2] Novel bioluminescent assay of alkaline phosphatase using adenosine-3′-phosphate-5′-phosphosulfate as substrate and the luciferin-luciferase reaction and its application
    Arakawa, H
    Shiokawa, M
    Imamura, O
    Maeda, M
    [J]. ANALYTICAL BIOCHEMISTRY, 2003, 314 (02) : 206 - 211
  • [3] ENZYMATICALLY AMPLIFIED TIME-RESOLVED FLUORESCENCE IMMUNOASSAY WITH TERBIUM CHELATES
    CHRISTOPOULOS, TK
    DIAMANDIS, EP
    [J]. ANALYTICAL CHEMISTRY, 1992, 64 (04) : 342 - 346
  • [4] Structural and photoluminescence properties of excited state intramolecular proton transfer capable compounds - Potential emissive and electron transport materials
    Gaenko, Alexander V.
    Devarajan, Ajitha
    Tselinskii, Igor V.
    Ryde, Ulf
    [J]. JOURNAL OF PHYSICAL CHEMISTRY A, 2006, 110 (25) : 7935 - 7942
  • [5] FLUOROMETRIC ANALYSIS OF HEAT-STABLE ALKALINE-PHOSPHATASE ACTIVITY IN HUMAN SERUM BY MEANS OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    ICHINOSE, N
    BABA, S
    NAKATSUJI, T
    NISHINO, N
    [J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1993, 345 (8-9): : 610 - 612
  • [6] DETERMINATION OF SERUM ALKALINE-PHOSPHATASE ACTIVITY BY ELECTROCHEMICAL DETECTION WITH FLOW-INJECTION ANALYSIS
    JACKSON, SD
    HALSALL, HB
    PESCE, AJ
    HEINEMAN, WR
    [J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1993, 346 (6-9): : 859 - 862
  • [7] Oxadiazole-containing material with intense blue phosphorescence emission for organic light-emitting diodes
    Liang, FS
    Wang, LX
    Ma, DG
    Jing, XB
    Wang, FS
    [J]. APPLIED PHYSICS LETTERS, 2002, 81 (01) : 4 - 6
  • [8] 4-amino-1-naphthylphosphate as a substrate for the amperometric detection of alkaline phosphatase activity and its application for immunoassay
    Másson, M
    Rúnarsson, OV
    Jóhannson, F
    Aizawa, M
    [J]. TALANTA, 2004, 64 (01) : 174 - 180
  • [9] T3P® (propylphosphonic anhydride) mediated conversion of carboxylic acids into acid azides and one-pot synthesis of ureidopeptides
    Narendra, Basavaprabhu N.
    Lamani, Ravi S.
    Sureshbabu, Vommina V.
    [J]. TETRAHEDRON LETTERS, 2010, 51 (22) : 3002 - 3005
  • [10] Ouyang J., 2004, J HETEROCYCLIC CHEM, V41, P359
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