Anti-LRP/LR-Specific Antibody IgG1-iS18 Significantly Reduces Adhesion and Invasion of Metastatic Lung, Cervix, Colon and Prostate Cancer Cells

被引：47

作者：

Omar, Aadilah ^{[1
]}

Reusch, Uwe ^{[2
]}

Knackmuss, Stefan ^{[2
]}

Little, Melvyn ^{[2
]}

Weiss, Stefan F. T. ^{[1
]}

机构：

[1] Univ Witwatersrand, Sch Mol & Cell Biol, ZA-2050 Johannesburg, South Africa

[2] Affimed Therapeut AG, D-69120 Heidelberg, Germany

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2012年 / 419卷 / 1-2期

基金：

新加坡国家研究基金会; 英国医学研究理事会;

关键词：

37-kDa/67-kDa laminin receptor; metastatic cancer; adhesion; invasion; therapy; 67-KDA LAMININ-RECEPTOR; BINDING PROTEIN; EXPRESSION; CARCINOMAS;

D O I：

10.1016/j.jmb.2012.02.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 37-kDa/67-kDa laminin receptor [laminin receptor precursor/high-affinity laminin receptor (LRP/LR)] is thought to play a major role in invasion and adhesion, key components of metastatic cancer. Lung cancer, cervical cancer, colon cancer and prostate cancer are among the top 10 cancer types worldwide. Here, we report that LRP/LR levels on the surface of lung cancer cells, cervical cancer cells, colon cancer cells and prostate cancer cells are significantly increased compared to non-tumorigenic fibroblasts. Adhesion of lung cancer cells, cervical cancer cells, colon cancer cells and prostate cancer cells to laminin-1 is significantly reduced, employing the anti-LRP/LR-specific antibody IgG1-iS18. Invasion of these cell lines into the Matrigel (TM) matrix was significantly impeded with IgG1-iS18. The Pearson's correlation coefficient proves a correlation between LRP/LR cell-surface levels and invasion potential, as well as adhesion and invasion, respectively. Our findings suggest that IgG1-iS18 antibody might act as alternative therapeutic tool for treatment of various metastatic cancer types. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：102 / 109

页数：8

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