Male age is associated with sperm DNA/chromatin integrity

被引:34
作者
Mettler, Anupama Deenadayal [1 ]
Govindarajan, Mirudhubashini [2 ]
Srinivas, Sapna [1 ]
Mithraprabhu, Sridurga [2 ,3 ]
Evenson, Donald [4 ]
Mahendran, Tara [5 ]
机构
[1] Infertil Inst & Res Ctr, Secunderabad, India
[2] Womens Ctr, Coimbatore, Tamil Nadu, India
[3] Monash Univ, Alfred Hosp, Australian Ctr Blood Dis, Melbourne, Vic, Australia
[4] SCSA Diagnost Inc, Brookings, SD USA
[5] Androl Ctr, 1056-2 Fortune Bldg,Avinashi Rd, Coimbatore 641018, Tamil Nadu, India
关键词
DNA fragmentation index; India; infertility; SCSA; CHROMATIN-STRUCTURE ASSAY; IN-VITRO FERTILIZATION; DNA FRAGMENTATION; SEMEN PARAMETERS; ACRIDINE-ORANGE; STRAND BREAKS; MALE PARTNERS; DAMAGE; TOOL; MEN;
D O I
10.1080/13685538.2019.1600496
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The diagnosis of sperm DNA integrity is increasingly recognized as being crucial to inform the clinical course in infertile couples. An internationally accepted sperm DNA fragmentation assay that determines the proportion of sperm and degree of broken sperm nuclear DNA with recognised clinical thresholds for identifying men at risk of infertility is the Sperm Chromatin Structure Assay (SCSA (R)). In this study, SCSA (R) test was utilised to evaluate the relevance of male age on sperm DNA quality in total of 6881 males of Indian origin. Analysis of proportions of DNA fragmentation index (%DFI) and high DNA stainability (%HDS) was performed based on four groups (<35, 35-40, 40-45, and >45 years of age). The impact of increasing male age on %DFI revealed that males >45 years of age had the highest %DFI and lowest %HDS compared to all other age groups (p<.001). This study is the largest population study and first of its kind in India that utilises SCSA (R) to assess the relevance of %DFI and %HDS to increasing age with potentially important implications for the choice of clinical course based on age and sperm quality of infertile males in India.
引用
收藏
页码:822 / 829
页数:8
相关论文
共 39 条
[21]  
Kumar N, 2017, INT J REPROD BIOMED, V15, P497
[22]   Analysis of human sperm DNA fragmentation index (DFI) related factors: a report of 1010 subfertile men in China [J].
Lu, Jin-Chun ;
Jing, Jun ;
Chen, Li ;
Ge, Yi-Feng ;
Feng, Rui-Xiang ;
Liang, Yuan-Jiao ;
Yao, Bing .
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2018, 16
[23]  
Marimuthu P, 2003, ASIAN J ANDROL, V5, P221
[24]  
Menezo Y, 2009, FERTIL STERIL, V92, ps213
[25]   Evaluation of sperm DNA structure, fragmentation and decondensation: an essential tool in the assessment of male infertility [J].
Menezo, Yves ;
Clement, Patrice ;
Amar, Edouard .
TRANSLATIONAL ANDROLOGY AND UROLOGY, 2017, 6 :S553-S556
[26]   Semen quality and age-specific changes: a study between two decades on 3,729 male partners of couples with normal sperm count and attending an andrology laboratory for infertility-related problems in an Indian city [J].
Mukhopadhyay, Dyutiman ;
Varghese, Alex C. ;
Pal, Manisha ;
Banerjee, Sudip K. ;
Bhattacharyya, Asok K. ;
Sharma, Rakesh K. ;
Agarwal, Ashok .
FERTILITY AND STERILITY, 2010, 93 (07) :2247-2254
[27]   Effect of Sperm DNA Fragmentation on Clinical Outcome of Frozen-Thawed Embryo Transfer and on Blastocyst Formation [J].
Ni, Wuhua ;
Xiao, Shiquan ;
Qiu, Xiufang ;
Jin, Jianyuan ;
Pan, Chengshuang ;
Li, Yan ;
Fei, Qianjin ;
Yang, Xu ;
Zhang, Liya ;
Huang, Xuefeng .
PLOS ONE, 2014, 9 (04)
[28]   Sperm chromatin structure assay in prediction of invitro fertilization outcome [J].
Oleszczuk, K. ;
Giwercman, A. ;
Bungum, M. .
ANDROLOGY, 2016, 4 (02) :290-296
[29]   Ethnicity is an independent predictor of IVF-ICSI outcome: a study of 5,549 cycles in Spain and India [J].
Patel, Azadeh P. ;
Patel, Jayesh A. ;
Cruz, Maria ;
Gupte-Shah, Arati ;
Garcia Velasco, Juan A. ;
Banker, Manish R. .
GYNECOLOGICAL ENDOCRINOLOGY, 2016, 32 (10) :819-822
[30]   Sperm DNA fragmentation in the total and vital fractions before and after density gradient centrifugation: Significance in male fertility diagnosis [J].
Punjabi, U. ;
Van Mulders, H. ;
Goovaerts, I ;
Peeters, K. ;
Clasen, K. ;
Janssens, P. ;
Zemtsova, O. ;
De Neubourg, D. .
CLINICAL BIOCHEMISTRY, 2018, 62 :47-54