Identification and Validation of Gene Expression Pattern and Signature in Patients with Immune Thrombocytopenia

被引：11
作者：
Ye, Qi-dong [1 ]
Jiang, Hui [1 ]
Liao, Xue-lian [1 ]
Chen, Kai [1 ]
Li, Shan-shan [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Shanghai Childrens Hosp, Dept Hematol, 355 Luding Rd, Shanghai 200062, Peoples R China
来源：
SLAS DISCOVERY | 2017年 / 22卷 / 02期
关键词：
immune thrombocytopenia; gene expression profile; pathway prediction; cytokine; SYSTEMIC-LUPUS-ERYTHEMATOSUS; PURPURA; CHILDREN; TRANSCRIPTOME; EPIDEMIOLOGY; CYTOKINES; HLA-DRB5; DISEASE; PROFILE; RISK;
D O I：
10.1177/1087057116664029
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In the present study, we sought to define genes associated with immune thrombocytopenia (ITP). Microarray analysis revealed that of 1002 genes associated with ITP, 309 genes had downregulated expression and 693 genes had upregulated expression in patients with ITP. Gene set enrichment analysis revealed that 11 pathways were positively correlated to ITP, such as type I diabetes mellitus, intestinal immune network for IgA production, and oxidative phosphorylation. The messenger RNA expression levels of the indicated genes, including HLA-DRB5, IGHV3-66, IFI27, FAM212A, PLD5, tumor necrosis factor (TNF)-alpha, interferon-gamma, interleukin (IL)-1 beta, and IL-4, were significantly increased in patients with ITP compared with healthy humans, while MMP8, SLC1A3, CRISP3, THBS1, FMN1, and IL-10 were decreased. In conclusion, the gene expression profile of patients with ITP has established a foundation to study the gene mechanism of ITP progression.
引用
收藏
页码：187 / 195
页数：9
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