Separation of steroid isomers by ion mobility mass spectrometry

被引：75

作者：

Ahonen, Linda ^{[1
]}

Fasciotti, Maira ^{[2
,3
]}

af Gennas, Gustav Boije ^{[1
]}

Kotiaho, Tapio ^{[1
,4
]}

Daroda, Romeu J. ^{[3
]}

Eberlin, Marcos ^{[2
]}

Kostiainen, Risto ^{[1
]}

机构：

[1] Univ Helsinki, Fac Pharm, Div Pharmaceut Chem, FI-00014 Helsinki, Finland

[2] Univ Estadual Campinas, ThoMSon Mass Spectrometry Lab, Inst Chem, UNICAMP, BR-13083970 Campinas, SP, Brazil

[3] INMETRO, Natl Inst Metrol Qual & Technol, Div Chem, BR-25250020 Duque De Caixas, RJ, Brazil

[4] Univ Helsinki, Dept Chem, Analyt Chem Lab, SF-00100 Helsinki, Finland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1310卷

基金：

芬兰科学院;

关键词：

Ion mobility mass spectrometry; Steroids; Steroid isomers; Derivatization; Collision cross section; ANABOLIC-ANDROGENIC STEROIDS; CHROMATOGRAPHY/MASS SPECTROMETRY; HUMAN BRAIN; HUMAN SERUM; NEUROSTEROIDS; TESTOSTERONE; GAS; METABOLITES; IONIZATION; ESTROGEN;

D O I：

10.1016/j.chroma.2013.08.056

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ion mobility mass spectrometry performed in a compact traveling wave cell (TWIM-MS) is shown to provide a reliable, fast and repeatable method to separate derivatized steroid isomers. Three steroid isomer pairs were analyzed in their native form and as their p-toluenesulfonyl isocyanate derivatives. The native steroids were separated from each other, but no separation could be attained for the isomers. The derivatized steroid isomers were, however, properly separated by TWIM-MS with peak-to-peak resolutions close to or as high as baseline resolution (Rp-p = 0.77-1.08). (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：133 / 137

页数：5

共 39 条

[1] Comparison of liquid chromatography-microchip/mass spectrometry to conventional liquid chromatography-mass spectrometry for the analysis of steroids [J].

Ahonen, Linda ;

Keski-Rahkonen, Pekka ;

Saarelainen, Taija ;

Paviala, Jenni ;

Ketola, Raimo A. ;

Auriola, Seppo ;

Poutanen, Matti ;

Kostiainen, Risto .

ANALYTICA CHIMICA ACTA, 2012, 721 :115-121

[2] Analysis of estrogens in serum and plasma from postmenopausal women: Past present, and future [J].

Blair, Ian A. .

STEROIDS, 2010, 75 (4-5) :297-306

[3] The coming of age of liquid chromatography coupled to tandem mass spectrometry in the endocrinology laboratory [J].

Carvalho, Valdemir Melechco .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2012, 883 :50-58

[4] Developments in ion mobility spectrometry-mass spectrometry [J].

Collins, DC ;

Lee, ML .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 372 (01) :66-73

[5]

Cowan DA, 1997, CLIN CHEM, V43, P1110

[6] Ion mobility spectrometry: a review. Part 1. Structural analysis by mobility measurement [J].

Creaser, CS ;

Griffiths, JR ;

Bramwell, CJ ;

Noreen, S ;

Hill, CA ;

Thomas, CLP .

ANALYST, 2004, 129 (11) :984-994

[7] Sites of metabolic substitution: investigating metabolite structures utilising ion mobility and molecular modelling [J].

Dear, Gordon J. ;

Munoz-Muriedas, Jordi ;

Beaumont, Claire ;

Roberts, Andrew ;

Kirk, Jayne ;

Williams, Jonathan P. ;

Campuzano, Iain .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2010, 24 (21) :3157-3162

[8] COMPARISON OF ION MOBILITY CONSTANTS OF SELECTED DRUGS AFTER CAPILLARY GAS-CHROMATOGRAPHY AND CAPILLARY SUPERCRITICAL FLUID CHROMATOGRAPHY [J].

EATHERTON, RL ;

MORRISSEY, MA ;

HILL, HH .

ANALYTICAL CHEMISTRY, 1988, 60 (20) :2240-2243

[9] Evaluating the utility of ion mobility separation in combination with high-pressure liquid chromatography/mass spectrometry to facilitate detection of trace impurities in formulated drug products [J].

Eckers, Christine ;

Laures, Alice M. -F. ;

Giles, Kevin ;

Major, Hilary ;

Pringle, Steve .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007, 21 (07) :1255-1263

[10] Current concepts in anabolic-androgenic steroids [J].

Evans, NA .

AMERICAN JOURNAL OF SPORTS MEDICINE, 2004, 32 (02) :534-542

← 1 2 3 4 →