The application of silk on tissue engineering scaffolds has been studied intensively because silk has an electrospinning technique using a good blood compatibility, excellent cytocompatibility and biodegradability. Silk consists of two polymers, fibroin and sericin. In spite of importance of sericin, most studies were focused on the fibroin only and the effect of residual sericin on the electrospinning performance of silk has not been considered. In this study, regenerated silk with different residual sericin contents was prepared by controlling the degumming conditions. The effects of the degumming conditions on the solution properties and electrospinning performance of silk were examined. The fast protein liquid chromatography (FPLC) measurements confirmed that the molecular weight of the regenerated silk decreased slightly with increasing residual sericin content. More molecular aggregation of silk occurred with increasing sericin content, resulting in an increase in the solution turbidity of formic acid. All silk formic acid solutions exhibited almost Newtonian fluid behavior and the viscosity increased with increasing sericin content. Interestingly, the dope solution viscosity of silk increased remarkably at sericin contents <1% (or degumming ratio >25%) leading to significant improvements in electrospinnability and an increase in the fiber diameter of the silk web. (C) 2013 Elsevier B.V. All rights reserved.
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Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151742, South KoreaSeoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151742, South Korea
Baek, Doo Hyun
;
Ki, Chang Seok
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机构:Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151742, South Korea
Ki, Chang Seok
;
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Um, In Chul
;
Park, Young Hwan
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机构:Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151742, South Korea
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Kolon Ind Inc, Appl Mat Res Inst, Gumi 730030, South KoreaKyungpook Natl Univ, Dept Biofibers & Mat Sci, Taegu 702701, South Korea
Cho, Hee Jung
;
Ki, Chang Seok
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Amorepacific Corp, R&D Ctr, Yongin 446729, South KoreaKyungpook Natl Univ, Dept Biofibers & Mat Sci, Taegu 702701, South Korea
Ki, Chang Seok
;
Oh, Hanjin
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Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151921, South KoreaKyungpook Natl Univ, Dept Biofibers & Mat Sci, Taegu 702701, South Korea
Oh, Hanjin
;
Lee, Ki Hoon
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Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151921, South KoreaKyungpook Natl Univ, Dept Biofibers & Mat Sci, Taegu 702701, South Korea
机构:
Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151742, South KoreaSeoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151742, South Korea
Baek, Doo Hyun
;
Ki, Chang Seok
论文数: 0引用数: 0
h-index: 0
机构:Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151742, South Korea
Ki, Chang Seok
;
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h-index:
机构:
Um, In Chul
;
Park, Young Hwan
论文数: 0引用数: 0
h-index: 0
机构:Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151742, South Korea
机构:
Kolon Ind Inc, Appl Mat Res Inst, Gumi 730030, South KoreaKyungpook Natl Univ, Dept Biofibers & Mat Sci, Taegu 702701, South Korea
Cho, Hee Jung
;
Ki, Chang Seok
论文数: 0引用数: 0
h-index: 0
机构:
Amorepacific Corp, R&D Ctr, Yongin 446729, South KoreaKyungpook Natl Univ, Dept Biofibers & Mat Sci, Taegu 702701, South Korea
Ki, Chang Seok
;
Oh, Hanjin
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151921, South KoreaKyungpook Natl Univ, Dept Biofibers & Mat Sci, Taegu 702701, South Korea
Oh, Hanjin
;
Lee, Ki Hoon
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151921, South KoreaKyungpook Natl Univ, Dept Biofibers & Mat Sci, Taegu 702701, South Korea