Effect of degumming condition on the solution properties and electrospinnablity of regenerated silk solution

被引:53
作者
Ko, Jae Sang [1 ]
Yoon, Kyunghwan [2 ]
Ki, Chang Seok [3 ]
Kim, Hyun Ju [4 ]
Bae, Do Gyu [4 ]
Lee, Ki Hoon [3 ]
Park, Young Hwan [3 ]
Um, In Chul [4 ]
机构
[1] Kolmar Korea Co Ltd, R&D Ctr, Yunki 339851, South Korea
[2] Washington Univ, Dept Biomed Engn, St Louis, MO 63130 USA
[3] Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 151921, South Korea
[4] Kyungpook Natl Univ, Dept Biofibers & Mat Sci, Taegu 702701, South Korea
基金
新加坡国家研究基金会;
关键词
Regenerated silk; Sericin; Solution properties; Electrospinning; BOMBYX-MORI SILK; STRUCTURAL CHARACTERISTICS; MOLECULAR-WEIGHT; FIBROIN NANOFIBERS; IN-VITRO; SERICIN; PROTEIN; WET; POLYMER; BIODEGRADATION;
D O I
10.1016/j.ijbiomac.2012.12.041
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The application of silk on tissue engineering scaffolds has been studied intensively because silk has an electrospinning technique using a good blood compatibility, excellent cytocompatibility and biodegradability. Silk consists of two polymers, fibroin and sericin. In spite of importance of sericin, most studies were focused on the fibroin only and the effect of residual sericin on the electrospinning performance of silk has not been considered. In this study, regenerated silk with different residual sericin contents was prepared by controlling the degumming conditions. The effects of the degumming conditions on the solution properties and electrospinning performance of silk were examined. The fast protein liquid chromatography (FPLC) measurements confirmed that the molecular weight of the regenerated silk decreased slightly with increasing residual sericin content. More molecular aggregation of silk occurred with increasing sericin content, resulting in an increase in the solution turbidity of formic acid. All silk formic acid solutions exhibited almost Newtonian fluid behavior and the viscosity increased with increasing sericin content. Interestingly, the dope solution viscosity of silk increased remarkably at sericin contents <1% (or degumming ratio >25%) leading to significant improvements in electrospinnability and an increase in the fiber diameter of the silk web. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:161 / 168
页数:8
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