Investigation of genetic susceptibility to Mycobacterium tuberculosis (VDR and IL10 genes) in a population with a high level of substructure in the Brazilian Amazon region

被引：9

作者：

Silva, C. A. ^{[1
]}

Fernandes, D. C. R. O. ^{[2
,3
]}

Braga, A. C. O. ^{[1
,3
]}

Cavalcante, G. C. ^{[2
]}

Sortica, V. A. ^{[3
,4
]}

Hutz, M. H. ^{[4
]}

Leal, D. F. V. B. ^{[3
]}

Fernades, M. R. ^{[3
]}

Santana-da-Silva, M. N. ^{[2
]}

Lopes Valente, S. E. ^{[3
]}

Pastana, L. F. ^{[3
]}

Pinto, P. D. C. ^{[2
,3
]}

Costa, G. E. ^{[3
]}

Ribeiro-dos-Santos, A. ^{[2
,3
]}

Santos, S. ^{[2
,3
]}

Santos, N. P. C. ^{[1
,2
,3
]}

机构：

[1] Fed Univ Para, Univ City Prof Jose Silveira Netto, Fac Med, 1 Augusto Correa St, BR-66075970 Belem, Para, Brazil

[2] Fed Univ Para, Univ City Prof Jose Silveira Netto, Lab Human & Med Genet, 1 Augusto Correa St, BR-66075970 Belem, Para, Brazil

[3] Univ Hosp Joao de Barros Barreto, Nucleus Oncol Res, High Complex Oncol Unit, 4487 Mundurucus St, BR-66073000 Belem, Para, Brazil

[4] Univ Fed Rio Grande do Sul, Dept Genet, 9500 Bento Goncalves Ave,Bldg 43323M,POB 15053, BR-91501970 Porto Alegre, RS, Brazil

来源：

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES | 2020年 / 98卷

关键词：

Mycobacterium tuberculosis; Genomic ancestry; Polymorphism; VDR; IL10; SLC11A1; MULTILOCUS GENOTYPE DATA; D-RECEPTOR GENE; HAPLOTYPE RECONSTRUCTION; PULMONARY TUBERCULOSIS; IL-10; POLYMORPHISMS; HOST GENETICS; NRAMP1; GENE; ASSOCIATION; INFERENCE; SLC11A1;

D O I：

10.1016/j.ijid.2020.06.090

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: Tuberculosis (TB) is an infectious and contagious disease that has been very influential in human history and presents high rates of mortality. The objective of this study was to investigate the association of VDR, IL10, and SLC11A1 gene polymorphisms with susceptibility to the presence of Mycobacterium tuberculosis infection. Methods: A total of 135 patients with confirmed TB and 141 healthy individuals were included in the analysis. Blood samples were collected for DNA extraction. Genotyping of the polymorphisms in the VDR and IL10 genes was performed by real-time PCR, and genotyping of the polymorphisms in the SLC11A1 gene by conventional PCR, followed by visualization in polyacrylamide gel. The genomic ancestry was obtained using an autosomal panel with 48 insertion/deletion ancestry-informative markers. Results: Polymorphisms TaqI (TT, p=0.004), Fold (CC and CC +CT, p = 0.012 and p=0.003, respectively), and Bsml (GG, p = 0.008) in the VDR gene, as well as A-592C (GC +AG, p=0.001) in the IL10 gene, were significantly associated with susceptibility to TB In addition, high production of VDR combined with low production of IL10 showed protection for the TB group (p = 0.035). Conclusions: The VDR polymorphisms may confer an increased risk and the IL10 haplotype may be a protection factor for the presence of M. tuberculosis infection in the Brazilian population. (C) 2020 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.

引用

页码：447 / 453

页数：7

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