The role of the temperature-regulated acyltransferase (PA3242) on growth, antibiotic resistance and virulence in Pseudomonas aeruginosa

被引:8
作者
Liang, Ying [1 ]
Guo, Zisheng [1 ]
Gao, Lang [1 ]
Guo, Qianqian [1 ]
Wang, Liyue [1 ]
Han, Yan [1 ]
Duan, Kangmin [1 ,2 ]
Shen, Lixin [1 ]
机构
[1] Northwest Univ, Fac Life Sci, Minist Educ, Key Lab Resources Biol & Biotechnol Western China, Xian 710069, Peoples R China
[2] Univ Manitoba, Fac Hlth Sci, Dept Oral Biol, 780 Bannatyne Ave, Winnipeg, MB R3E 0W2, Canada
关键词
Pseudomonas aeruginosa; Acyltransferase; Growth; Antibiotic resistance; Virulence; GRAM-NEGATIVE BACTERIA; LIPID-A BIOSYNTHESIS; ESCHERICHIA-COLI; EPITHELIAL-CELLS; YERSINIA-PESTIS; ACYL CHAINS; HTRB GENE; IV PILI; LIPOPOLYSACCHARIDE; ACYLATION;
D O I
10.1016/j.micpath.2016.09.019
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Pseudomonas aeruginosa (PAO1) is an important opportunistic pathogen that thrives in various environments. It is known that the structural variations of the lipopolysaccharide (LPS), including lipid A moiety play an important role in encountering environmental changes. Genes PA3242 and PA0011 have recently been reported to be responsible for secondary-acylation of lipid A in P. aeruginosa. In this study, we confirmed that the PA3242-dependant secondary acylation affects the growth, antibiotic resistance and virulence of PAO1 and functions as a more predominant acyltransferase than PA0011. PA3242 mutant showed inhibited growth at 37 degrees C and inviability at 28 degrees C in rich medium LB. The inactivation of PA3242 leads to more sensitivity to a wide range of antibiotics than PAO1(Delta PA0011). Moreover, the virulence of PAO1(Delta PA3242) was attenuated more significantly than that of PAO1 and PAO1(Delta PA0011). The outer membrane integrity and stability of PAO1(Delta PA3242) were seriously compromised. Furthermore, PAO1(Delta PA3242) lost most of pilus and exhibited severely damaged cell envelope, which is probably responsible for the deficiency of swimming, swarming and twitching. These results partially explained the decreased antibiotic resistance and attenuated virulence of PAO1(Delta PA3242) compared to PAO1(Delta PA0011) and PAO1. Our study demonstrated that PA3242-dependent secondary acylation of lipid A plays a predominant role in growth, antibiotic resistance and virulence of PAO1 than PA0011. (C) 2016 Published by Elsevier Ltd.
引用
收藏
页码:126 / 135
页数:10
相关论文
共 51 条
  • [1] Type IV pili interactions promote intercellular association and moderate swarming of Pseudomonas aeruginosa
    Anyan, Morgen E.
    Amiri, Aboutaleb
    Harvey, Cameron W.
    Tierra, Giordano
    Morales-Soto, Nydia
    Driscoll, Callan M.
    Alber, Mark S.
    Shrout, Joshua D.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (50) : 18013 - 18018
  • [2] Genomic profiling of iron-responsive genes in Salmonella enterica serovar typhimurium by high-throughput screening of a random promoter library
    Bjarnason, J
    Southward, CM
    Surette, MG
    [J]. JOURNAL OF BACTERIOLOGY, 2003, 185 (16) : 4973 - 4982
  • [3] Reinforcing Lipid A Acylation on the Cell Surface of Acinetobacter baumannii Promotes Cationic Antimicrobial Peptide Resistance and Desiccation Survival
    Boll, Joseph M.
    Tucker, Ashley T.
    Klein, Dustin R.
    Beltran, Alexander M.
    Brodbelt, Jennifer S.
    Davies, Bryan W.
    Trent, M. Stephen
    [J]. MBIO, 2015, 6 (03): : 1 - 11
  • [4] Pseudomonas aeruginosa Twitching Motility: Type IV Pili in Action
    Burrows, Lori L.
    [J]. ANNUAL REVIEW OF MICROBIOLOGY, VOL 66, 2012, 66 : 493 - 520
  • [5] Effect of cold shock on lipid A biosynthesis in Escherichia coli -: Induction at 12°C of an acyltransferase specific for palmitoleoyl-acyl carrier protein
    Carty, SM
    Sreekumar, KR
    Raetz, CRH
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (14) : 9677 - 9685
  • [6] Characterization of a cryptic plasmid from Pseudomonas sp and utilization of its temperature-sensitive derivatives for genetic manipulation
    Chen, Lin
    Wang, Wenli
    Sun, Weijie
    Surette, Michael
    Duan, Kangmin
    [J]. PLASMID, 2010, 64 (02) : 110 - 117
  • [7] Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides
    Clements, Abigail
    Tull, Dedreia
    Jenney, Adam W.
    Farn, Jacinta L.
    Kim, Sang-Hyun
    Bishop, Russell E.
    McPhee, Joseph B.
    Hancock, Robert E. W.
    Hartland, Elizabeth L.
    Pearse, Martin J.
    Wijburg, Odilia L. C.
    Jackson, David C.
    McConville, Malcolm J.
    Strugnell, Richard A.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (21) : 15569 - 15577
  • [8] Function of the htrB high temperature requirement gene of Escherichia coli in the acylation of lipid A - HtrB catalyzed incorporation of laurate
    Clementz, T
    Bednarski, JJ
    Raetz, CRH
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (20) : 12095 - 12102
  • [9] Two msbB genes encoding maximal acylation of lipid A are required for invasive Shigella flexneri to mediate inflammatory rupture and destruction of the intestinal epithelium
    d'Hauteville, H
    Khan, S
    Maskell, DJ
    Kussak, A
    Weintraub, A
    Mathison, J
    Ulevitch, RJ
    Wuscher, N
    Parsot, C
    Sansonetti, PJ
    [J]. JOURNAL OF IMMUNOLOGY, 2002, 168 (10) : 5240 - 5251
  • [10] BROAD HOST RANGE DNA CLONING SYSTEM FOR GRAM-NEGATIVE BACTERIA - CONSTRUCTION OF A GENE BANK OF RHIZOBIUM-MELILOTI
    DITTA, G
    STANFIELD, S
    CORBIN, D
    HELINSKI, DR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12): : 7347 - 7351