Use and Abuse of RNAi to Study Mammalian Gene Function

被引:120
作者
Kaelin, William G., Jr. [1 ]
机构
[1] Dana Farber Canc Inst, Dept Adult Oncol, Off M457, Boston, MA 02215 USA
关键词
HUMAN-CELLS; SCREENS; INTERFERENCE; EXPRESSION;
D O I
10.1126/science.1225787
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Realizing the full potential of si/shRNA technology requires more sophisticated approaches to address the pitfalls.
引用
收藏
页码:421 / 422
页数:2
相关论文
共 15 条
  • [1] Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1
    Barbie, David A.
    Tamayo, Pablo
    Boehm, Jesse S.
    Kim, So Young
    Moody, Susan E.
    Dunn, Ian F.
    Schinzel, Anna C.
    Sandy, Peter
    Meylan, Etienne
    Scholl, Claudia
    Froehling, Stefan
    Chan, Edmond M.
    Sos, Martin L.
    Michel, Kathrin
    Mermel, Craig
    Silver, Serena J.
    Weir, Barbara A.
    Reiling, Jan H.
    Sheng, Qing
    Gupta, Piyush B.
    Wadlow, Raymond C.
    Le, Hanh
    Hoersch, Sebastian
    Wittner, Ben S.
    Ramaswamy, Sridhar
    Livingston, David M.
    Sabatini, David M.
    Meyerson, Matthew
    Thomas, Roman K.
    Lander, Eric S.
    Mesirov, Jill P.
    Root, David E.
    Gilliland, D. Gary
    Jacks, Tyler
    Hahn, William C.
    [J]. NATURE, 2009, 462 (7269) : 108 - U122
  • [2] Factors Affecting Reproducibility between Genome-Scale siRNA-Based Screens
    Barrows, Nicholas J.
    Le Sommer, Caroline
    Garcia-Blanco, Mariano A.
    Pearson, James L.
    [J]. JOURNAL OF BIOMOLECULAR SCREENING, 2010, 15 (07) : 735 - 747
  • [3] Raise standards for preclinical cancer research
    Begley, C. Glenn
    Ellis, Lee M.
    [J]. NATURE, 2012, 483 (7391) : 531 - 533
  • [4] TAL Effectors: Customizable Proteins for DNA Targeting
    Bogdanove, Adam J.
    Voytas, Daniel F.
    [J]. SCIENCE, 2011, 333 (6051) : 1843 - 1846
  • [5] A system for stable expression of short interfering RNAs in mammalian cells
    Brummelkamp, TR
    Bernards, R
    Agami, R
    [J]. SCIENCE, 2002, 296 (5567) : 550 - 553
  • [6] Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens
    Carette, Jan E.
    Guimaraes, Carla P.
    Varadarajan, Malini
    Park, Annie S.
    Wuethrich, Irene
    Godarova, Alzbeta
    Kotecki, Maciej
    Cochran, Brent H.
    Spooner, Eric
    Ploegh, Hidde L.
    Brummelkamp, Thijn R.
    [J]. SCIENCE, 2009, 326 (5957) : 1231 - 1235
  • [7] Minimizing the risk of reporting false positives in large-scale RNAi screens
    Echeverri, Christophe J.
    Beachy, Philip A.
    Baum, Buzz
    Boutros, Michael
    Buchholz, Frank
    Chanda, Sumit K.
    Downward, Julian
    Ellenberg, Jan
    Fraser, Andrew G.
    Hacohen, Nir
    Hahn, William C.
    Jackson, Aimee L.
    Kiger, Amy
    Linsley, Peter S.
    Lum, Lawrence
    Ma, Yong
    Mathey-Prevot, Bernard
    Root, David E.
    Sabatini, David M.
    Taipale, Jussi
    Perrimon, Norbert
    Bernards, Rene
    [J]. NATURE METHODS, 2006, 3 (10) : 777 - 779
  • [8] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    Elbashir, SM
    Harborth, J
    Lendeckel, W
    Yalcin, A
    Weber, K
    Tuschl, T
    [J]. NATURE, 2001, 411 (6836) : 494 - 498
  • [9] Knockdown Screens to Knockout HIV-1
    Goff, Stephen P.
    [J]. CELL, 2008, 135 (03) : 417 - 420
  • [10] AAV-mediated gene targeting methods for human cells
    Khan, Iram F.
    Hirata, Roli K.
    Russell, David W.
    [J]. NATURE PROTOCOLS, 2011, 6 (04) : 482 - 501