Two amino acid residues in the matrix protein M1 contribute to the virulence difference of H5N1 avian influenza viruses in mice

被引：219

作者：

Fan, Shufang ^{[1
,2
]}

Deng, Guohua ^{[1
,2
]}

Song, Jiasheng ^{[1
,2
]}

Tian, Guobin ^{[1
,2
]}

Suo, Yongbing ^{[1
,2
]}

Jiang, Yongping ^{[1
,2
]}

Guan, Yuntao ^{[1
,2
]}

Bu, Zhigao ^{[1
,2
]}

Kawaola, Yoshihiro ^{[3
,4
]}

Chen, Hualan ^{[1
,2
]}

机构：

[1] CAAS, Anim Influenza Lab, Minist Agr, Harbin Vet Res Inst, 427 Maduan St, Harbin 150001, Peoples R China

[2] CAAS, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

[3] Univ Tokyo, Inst Med Sci, Tokyo 1088639, Japan

[4] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

来源：

VIROLOGY | 2009年 / 384卷 / 01期

关键词：

Avian influenza viruses; A VIRUS; NUCLEAR TRANSPORT; MOLECULAR-BASIS; MOUSE LUNG; RNA; RIBONUCLEOPROTEINS; MUTATIONS; IDENTIFICATION; HEMAGGLUTININ; INHIBITION;

D O I：

10.1016/j.virol.2008.11.044

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A/duck/Guangxi/53/2002 (DKGX/53) and A/duck/Fujian/01/2002 (DKFJ/01) are H5N1 avian influenza viruses that are lethal in chickens. In mice, however, DKFJ/01 is highly pathogenic, whereas DKGX/53 displays low pathogenicity. In this study, we used reverse genetics to demonstrate that two amino acid residues at positions 30 and 215 of the M1 protein of these two viruses are important determinants for pathogenicity in mice. We thus firstly prove the M1 protein contributes to the virulence of H5N1 viruses in mice, and the amino acid residues shown to attenuate the virulence could be targeted in influenza virus candidates for live vaccine development. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：28 / 32

页数：5

共 28 条

[1] STRUCTURE OF INFLUENZA-VIRUS RNP .1. INFLUENZA-VIRUS NUCLEOPROTEIN MELTS SECONDARY STRUCTURE IN PANHANDLE RNA AND EXPOSES THE BASES TO THE SOLVENT [J].